Re: [Histonet] Von Kossa stain kit
Thought of another question! What other vendors do people order special stain kits? We have been using Poly Scientific, which is very good, but costly. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Von Kossa stain kit
It has been my experience that you do not need to acid wash the glassware for the silver nitrate step. The only critical parts are the time (5 minutes) in silver nitrate (protected from light), the 3 dH2O rinses (also protected from light), developing time (2 minutes) in sodium carbonate-formaldehyde solution (protected from light), 2 dH2O rinses (no need to protect from light), the 30 seconds in Farmer's Diminisher (sodium thiosulfate-potassium ferricyanide solution) and tap water rinse. The sodium-carbonate formaldehyde solution is use to chemically develop the silver attachment to any presence of mineralization. Most people use natural light, but the chemical step is more consistent in my opinion. Additionally, since the silver nitrate step is used to attach silver ions to the presence of minerals, the use of any metal is prohibited as it will also bind silver. However, if you use plastic, glass, or even stainless steel, no worries at all. As for a reliable kit, I use the kit from Dorn and Hart Microedge (www.dornandhart.com). You can also choose you preferred counterstain with this kit. I routinely work with mineralized bone and therefore use the Von Kossa - MacNeal's Tetrachrome stain kit. Hope this helps! Best, Jack Date: Tue, 4 Oct 2011 11:02:45 -0400 From: msherw...@partners.org To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Von Kossa stain kit Thought of another question! What other vendors do people order special stain kits? We have been using Poly Scientific, which is very good, but costly. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Re: [Histonet] Von Kossa stain kit
I've used Rowley Biochemical for years: http://rowleybio.com/ Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, October 04, 2011 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Von Kossa stain kit Thought of another question! What other vendors do people order special stain kits? We have been using Poly Scientific, which is very good, but costly. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
