Re: [Histonet] Warthin Starry Help
Although many histotechs successfully use the Warhin Starry stain (or so they say!) I find it the most unreliable and capricious of all stains, so much so that I stopped using it many years ago and started using the modified Steiner that stains H.pylory and all Treponema and related bacteria. The only alleged drawback of the Steiner stain is obtaining uranyl nitrate and its low radioactivity. To eliminate that problem I substituted it with phosphotungstic solution. Under separate cover I am sending you my modified Steiner method with phosphotungstic acid. Another thing, I commend you for preparing your own reagents. You belong to a dying breed of histotechs. René J. From: Campbell, Tasha M. tmcampb...@fmh.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, January 14, 2014 9:55 AM Subject: [Histonet] Warthin Starry Help I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. The tissue will stain normally with the yellow to brown background and I the developing solution turns black when it is left on paper but the h.pylori organisms are not staining. I am getting very frustrated and need some help please. Thank you. Tasha Campbell, HTL tmcampb...@fmh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Warthin Starry Help
Hydroquinone has a short shelf life once opened. On Jan 14, 2014, at 6:59 AM, Campbell, Tasha M. tmcampb...@fmh.org wrote: I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. The tissue will stain normally with the yellow to brown background and I the developing solution turns black when it is left on paper but the h.pylori organisms are not staining. I am getting very frustrated and need some help please. Thank you. Tasha Campbell, HTL tmcampb...@fmh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Warthin Starry Help
I was wondering about that. Even in its crystal form? I've been trying to test every possible problem, though, and I am using a kit right now so I have substituted the kit's hydroquinone in place of mine and used the rest of my reagents. It still didn't work. So this has to mean there is another problem. But with the entire kit's reagents it works. I have narrowed down by substituting my reagents in for the kit's one by one and the only combination I can get to work is using my 1% silver for impregnation and then the kit's reagents for developing so this has to mean that my silver is good. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: Jennifer MacDonald [mailto:jmacdon...@mtsac.edu] Sent: Tuesday, January 14, 2014 10:16 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry Help Hydroquinone has a short shelf life once opened. On Jan 14, 2014, at 6:59 AM, Campbell, Tasha M. tmcampb...@fmh.org wrote: I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. The tissue will stain normally with the yellow to brown background and I the developing solution turns black when it is left on paper but the h.pylori organisms are not staining. I am getting very frustrated and need some help please. Thank you. Tasha Campbell, HTL tmcampb...@fmh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Warthin Starry Help
My hydroquonine was received in the lab in 1980 and it works. As long as my control is positive I am allowed to keep it but then I work in a veterinary diagnostic lab and am not under all the regulations that those of you in hospitals are under. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, January 14, 2014 10:16 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry Help Hydroquinone has a short shelf life once opened. On Jan 14, 2014, at 6:59 AM, Campbell, Tasha M. tmcampb...@fmh.org wrote: I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. The tissue will stain normally with the yellow to brown background and I the developing solution turns black when it is left on paper but the h.pylori organisms are not staining. I am getting very frustrated and need some help please. Thank you. Tasha Campbell, HTL tmcampb...@fmh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Warthin Starry Help
Same here. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Tuesday, January 14, 2014 9:53 AM To: Jennifer MacDonald; Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Warthin Starry Help My hydroquonine was received in the lab in 1980 and it works. As long as my control is positive I am allowed to keep it but then I work in a veterinary diagnostic lab and am not under all the regulations that those of you in hospitals are under. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, January 14, 2014 10:16 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry Help Hydroquinone has a short shelf life once opened. On Jan 14, 2014, at 6:59 AM, Campbell, Tasha M. tmcampb...@fmh.org wrote: I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. The tissue will stain normally with the yellow to brown background and I the developing solution turns black when it is left on paper but the h.pylori organisms are not staining. I am getting very frustrated and need some help please. Thank you. Tasha Campbell, HTL tmcampb...@fmh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
