RE: [Histonet] how to prevent foldings on femoral head cartilage tissue
Sometimes placing them on a hot plate at about 60C will help get out the folds, the paraffin needs to melt and the sections need to turn clear then take it off the hot plate. If you leave it too long on the hotplate the articular cartilage may fold over on itself. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia Sent: Thursday, June 17, 2010 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to prevent foldings on femoral head cartilage tissue Hello histonetters especailly hard tissue group I have a pig femoral head bone tissue embedded in paraffin and I have a hard time getting rid of the folding problem. I tried to remedy by lowering my temperature to 38 C and putting them in 5% alcohol before placing them in water bath I still have a lots of folding formation on some areas of the cartilage. Is there any other technique to remedy this problem. I appreciate your help. Thank you. reuel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] how to prevent foldings on femoral head cartilage tissue
Reuel, Do the opposite and turn up the temp on the waterbath and let the section float out a little before the wax starts to break up. You can even gently use the forcepts to tease out any folds and this will definitely help to release any wrinkles in the cartilage. Then, make sure the section is free of large droplets of water before transfer of the slide to the slide warmer. Next, let slide sit on slide warmer until the wax is melted and then bake the section for a few minutes at a higher temperature to firmly secure the section to the glass slide. Bob Skinner at UAMS showed me this technique at the beginning of the year and it works nicely, especially for even larger human femoral heads! Jack Date: Thu, 17 Jun 2010 12:10:10 -0500 From: reuel.corne...@tsrh.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to prevent foldings on femoral head cartilage tissue Hello histonetters especailly hard tissue group I have a pig femoral head bone tissue embedded in paraffin and I have a hard time getting rid of the folding problem. I tried to remedy by lowering my temperature to 38 C and putting them in 5% alcohol before placing them in water bath I still have a lots of folding formation on some areas of the cartilage. Is there any other technique to remedy this problem. I appreciate your help. Thank you. reuel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
