Re: [Histonet] new antibody lot
Ours are validated and optimized anew, just like if we were working up an entirely new antibody. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: anita dudley azdud...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, September 22, 2011 9:47 AM Subject: [Histonet] new antibody lot how are others handling new lot numbers of antibodies when you receive them in the lab? thanks for any input. have a great day!!! anita dudley providence hospital mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new antibody lot
We check out a couple of control tissues on one instrument only for new lots. If the new lot does not appear equivalent to the current lot and needs further optimization, we run it on all of our instruments. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Thursday, September 22, 2011 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new antibody lot how are others handling new lot numbers of antibodies when you receive them in the lab? thanks for any input. have a great day!!! anita dudley providence hospital mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] new antibody lot
We run 1 slide from each new lot, and compare it with the previous stained slide and have it signed off. We have several lot to lot slides which come in very handy for seeing how the antibodies will vary from lot to lot. On Thu, Sep 22, 2011 at 7:47 AM, anita dudley azdud...@hotmail.com wrote: how are others handling new lot numbers of antibodies when you receive them in the lab? thanks for any input. have a great day!!! anita dudley providence hospital mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new antibody lot
That is what we do as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie [foreig...@gmail.com] Sent: Thursday, September 22, 2011 1:18 PM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new antibody lot We run 1 slide from each new lot, and compare it with the previous stained slide and have it signed off. We have several lot to lot slides which come in very handy for seeing how the antibodies will vary from lot to lot. On Thu, Sep 22, 2011 at 7:47 AM, anita dudley azdud...@hotmail.com wrote: how are others handling new lot numbers of antibodies when you receive them in the lab? thanks for any input. have a great day!!! anita dudley providence hospital mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new antibody lot
same here. McMahon, Loralee A loralee_mcma...@urmc.rochester.edu 9/22/2011 1:43 PM That is what we do as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie [foreig...@gmail.com] Sent: Thursday, September 22, 2011 1:18 PM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new antibody lot We run 1 slide from each new lot, and compare it with the previous stained slide and have it signed off. We have several lot to lot slides which come in very handy for seeing how the antibodies will vary from lot to lot. On Thu, Sep 22, 2011 at 7:47 AM, anita dudley azdud...@hotmail.com wrote: how are others handling new lot numbers of antibodies when you receive them in the lab? thanks for any input. have a great day!!! anita dudley providence hospital mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new antibody lot
CAP has recommendations from the following paper Recommendations for Improved Standardization of Immunohistochemistry Neal S. Goldstein, MD, Stephen M. Hewitt, MD, PhD, Clive R. Taylor, MD, DPhil, Hadi Yaziji, MD, David G. Hicks, MD, and Members of Ad-Hoc Committee On Immunohistochemistry Standardization 3 tissues need to be evaluated - one that is strongly positive, one that is weak to moderately positive and one that is negative for the particular antibody. If you put these all on one slide then it would just be one slide. Liz From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley [azdud...@hotmail.com] Sent: Thursday, September 22, 2011 8:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new antibody lot how are others handling new lot numbers of antibodies when you receive them in the lab? thanks for any input. have a great day!!! anita dudley providence hospital mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new antibody lot
yes, they are recommendations. Unless it has changed, I believe it is up to the discretion of your Doctoral Director. Elizabeth Chlipala l...@premierlab.com 9/22/2011 2:07 PM CAP has recommendations from the following paper Recommendations for Improved Standardization of Immunohistochemistry Neal S. Goldstein, MD, Stephen M. Hewitt, MD, PhD, Clive R. Taylor, MD, DPhil, Hadi Yaziji, MD, David G. Hicks, MD, and Members of Ad-Hoc Committee On Immunohistochemistry Standardization 3 tissues need to be evaluated - one that is strongly positive, one that is weak to moderately positive and one that is negative for the particular antibody. If you put these all on one slide then it would just be one slide. Liz From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley [azdud...@hotmail.com] Sent: Thursday, September 22, 2011 8:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new antibody lot how are others handling new lot numbers of antibodies when you receive them in the lab? thanks for any input. have a great day!!! anita dudley providence hospital mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
