RE: [Histonet] saffron vs. safran du gatinais
We buy our alcoholic saffron from Rowley biochemical. Already made and reusable. We were still using it when it hit its expiration date. You can but all the Movat's reagents as a kit or but what you need. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Thursday, January 05, 2012 4:21 PM To: 'Villarreal, Beth'; Histonet Subject: RE: [Histonet] saffron vs. safran du gatinais Beth, In the past, people replied to Histonet with the suggestion to buy saffron aka safran du gatinais from a grocery store spice section. Depending on where you are located e.g. a bigger city, try to find a store that sells spices from India may have the freshest saffron. However, you won't suffer the sticker shock of buying it from a chemical supplier. It still tends to be expensive but not like chemical company prices.Once we made the alcoholic saffron solution for Movat's pentachrome, we stored this solution in a container with a dessicant to maintain a water free environment. I was fascinated that Tom actually grew and harvested saffron from the flowers. That is true devotion, but I suspect it was for those delicious sounding nubbies . Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Villarreal, Beth Sent: Thursday, January 05, 2012 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saffron vs. safran du gatinais Hello histonet, I have a protocol that calls for safran du gatinais and am experiencing some serious sticker shock. Can I substitute saffron in my solution or am I asking for trouble? Many thanks, Beth Beth Villarreal Scientist I Novartis Institutes for BioMedical Research, Inc. 300 Technology Square Cambridge, MA 02139 USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] saffron vs. safran du gatinais
Hi Beth, Saffron and safran du gatinais both refer to the dried stigma of the saffron crocus. They are both saffron. I use and alcoholic extract of Saffron to stain collagen in Movat stains. I've tried saffron from multiple sources and price definitely does not always correlate with quality. I gave up on Sigma-Aldrich, for example. The best saffron has a deep red color and is highly aromatic. There are possible workarounds for the lower quality material--like use more saffron, or stain longer, but best to use the best. What stain are you doing and what solution are you making with the saffron? Jerry Ricks Research Scientist University of Washington Department of Pathology From: beth.villarr...@novartis.com To: histonet@lists.utsouthwestern.edu Date: Thu, 5 Jan 2012 20:02:21 + Subject: [Histonet] saffron vs. safran du gatinais Hello histonet, I have a protocol that calls for safran du gatinais and am experiencing some serious sticker shock. Can I substitute saffron in my solution or am I asking for trouble? Many thanks, Beth Beth Villarreal Scientist I Novartis Institutes for BioMedical Research, Inc. 300 Technology Square Cambridge, MA 02139 USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] saffron vs. safran du gatinais
Beth, In the past, people replied to Histonet with the suggestion to buy saffron aka safran du gatinais from a grocery store spice section. Depending on where you are located e.g. a bigger city, try to find a store that sells spices from India may have the freshest saffron. However, you won't suffer the sticker shock of buying it from a chemical supplier. It still tends to be expensive but not like chemical company prices.Once we made the alcoholic saffron solution for Movat's pentachrome, we stored this solution in a container with a dessicant to maintain a water free environment. I was fascinated that Tom actually grew and harvested saffron from the flowers. That is true devotion, but I suspect it was for those delicious sounding nubbies . Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Villarreal, Beth Sent: Thursday, January 05, 2012 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saffron vs. safran du gatinais Hello histonet, I have a protocol that calls for safran du gatinais and am experiencing some serious sticker shock. Can I substitute saffron in my solution or am I asking for trouble? Many thanks, Beth Beth Villarreal Scientist I Novartis Institutes for BioMedical Research, Inc. 300 Technology Square Cambridge, MA 02139 USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
