RE: [Histonet] your opinion on microtome

2011-08-05 Thread Bea DeBrosse-Serra
My favorite is the Leica RM2255.

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
1896 Rutherford Road
Carlsbad, CA 92008
760-603-2371



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Friday, August 05, 2011 7:58 AM
To: amitapan...@torrentpharma.com; histonet@lists.utsouthwestern.edu; 
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] your opinion on microtome

We have a Reichert-Jung 820 manual (old old old) and a fully automated Microm 
HM 355.  Both of us who cut prefer the manual.

The manual is more compact, easier to clean, cheaper. 
The Microm HM 355 is twice as big and the blade holder - adjustment screw 
set-up is neither convenient or easy to optimize.

Bottom line, the fewer moving parts the better.

Tresa 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
amitapan...@torrentpharma.com
Sent: Friday, August 05, 2011 1:25 AM
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] your opinion on microtome

Hello friends,

I am planning to  buy a microtome for my lab i already have Leica 
manual one which is working perfectly.

Now I wish to introduce a fully motorized one. 

Short listed items are: Leica RM2255, Microm 355S, Shandon Finess ME.

Let me know your frank review on
1. manual versus automated option
2. Above all three items.in terms of their performance, maintenance and 
breakdown etc. or due you suggest any more better option


Thanks in advance for your feed back. In past also histonetters helped me 
to solve many problems, looking forward this time also.

Amita
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RE: [Histonet] your opinion on microtome

2011-08-05 Thread Marcum, Pamela A
We have 3 Leica RM2155s, 3 RM2255 and one Microm 355S.  We all love the Leica 
models and some prefer one or the other. I do find I am the only one to use the 
foot petal or most of the really automated features.  The Microm is a good 
microtome however; only person uses it daily and no one else seems to want to 
switch.  I have used the Thermo Millennium and it is a good microtome too.  I 
loved the old black AO I started on and still miss sometimes.  We have a 
Reichert Jung 2030 Rotary that also works very well.  I think it is what you 
are accustomed to using and the motorized microtomes while good are expensive 
and it you don't need or want the features it can be a waste of money. 

I do love the using the automated fine/coarse advance on the motorized as 
opposed to turning a wheel.  It is easier on the hands and wrists for 
repetitive motion injuries.  

Pam Marcum
UAMS
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Friday, August 05, 2011 9:58 AM
To: amitapan...@torrentpharma.com; histonet@lists.utsouthwestern.edu; 
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] your opinion on microtome

We have a Reichert-Jung 820 manual (old old old) and a fully automated Microm 
HM 355.  Both of us who cut prefer the manual.

The manual is more compact, easier to clean, cheaper. 
The Microm HM 355 is twice as big and the blade holder - adjustment screw 
set-up is neither convenient or easy to optimize.

Bottom line, the fewer moving parts the better.

Tresa 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
amitapan...@torrentpharma.com
Sent: Friday, August 05, 2011 1:25 AM
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] your opinion on microtome

Hello friends,

I am planning to  buy a microtome for my lab i already have Leica 
manual one which is working perfectly.

Now I wish to introduce a fully motorized one. 

Short listed items are: Leica RM2255, Microm 355S, Shandon Finess ME.

Let me know your frank review on
1. manual versus automated option
2. Above all three items.in terms of their performance, maintenance and 
breakdown etc. or due you suggest any more better option


Thanks in advance for your feed back. In past also histonetters helped me 
to solve many problems, looking forward this time also.

Amita
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RE: [Histonet] your opinion on microtome

2011-08-05 Thread Rene J Buesa
The same (the less moving parts the better) applies to any instrument or piece 
of equipment.
Remember the VW Beetle?
René J.

--- On Fri, 8/5/11, Goins, Tresa  wrote:


From: Goins, Tresa 
Subject: RE: [Histonet] your opinion on microtome
To: "amitapan...@torrentpharma.com" , 
"histonet@lists.utsouthwestern.edu" , 
"histonet-boun...@lists.utsouthwestern.edu" 

Date: Friday, August 5, 2011, 10:57 AM


We have a Reichert-Jung 820 manual (old old old) and a fully automated Microm 
HM 355.  Both of us who cut prefer the manual.

The manual is more compact, easier to clean, cheaper. 
The Microm HM 355 is twice as big and the blade holder - adjustment screw 
set-up is neither convenient or easy to optimize.

Bottom line, the fewer moving parts the better.

Tresa     


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
amitapan...@torrentpharma.com
Sent: Friday, August 05, 2011 1:25 AM
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] your opinion on microtome

Hello friends,

I am planning to  buy a microtome for my lab i already have Leica 
manual one which is working perfectly.

Now I wish to introduce a fully motorized one. 

Short listed items are: Leica RM2255, Microm 355S, Shandon Finess ME.

Let me know your frank review on
1. manual versus automated option
2. Above all three items.in terms of their performance, maintenance and 
breakdown etc. or due you suggest any more better option


Thanks in advance for your feed back. In past also histonetters helped me 
to solve many problems, looking forward this time also.

Amita
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RE: [Histonet] your opinion on microtome

2011-08-05 Thread Goins, Tresa
We have a Reichert-Jung 820 manual (old old old) and a fully automated Microm 
HM 355.  Both of us who cut prefer the manual.

The manual is more compact, easier to clean, cheaper. 
The Microm HM 355 is twice as big and the blade holder - adjustment screw 
set-up is neither convenient or easy to optimize.

Bottom line, the fewer moving parts the better.

Tresa 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
amitapan...@torrentpharma.com
Sent: Friday, August 05, 2011 1:25 AM
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] your opinion on microtome

Hello friends,

I am planning to  buy a microtome for my lab i already have Leica 
manual one which is working perfectly.

Now I wish to introduce a fully motorized one. 

Short listed items are: Leica RM2255, Microm 355S, Shandon Finess ME.

Let me know your frank review on
1. manual versus automated option
2. Above all three items.in terms of their performance, maintenance and 
breakdown etc. or due you suggest any more better option


Thanks in advance for your feed back. In past also histonetters helped me 
to solve many problems, looking forward this time also.

Amita
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