I do not bake my unstained slides for IHC controls and store them at 4dc. I bake them just before use. They do last longer this way but Richard is right, there is no telling because it all depends on the fixation, target protein stability and storage conditions. It is always a good idea to cut fresh sections if the stored unstained sections do not perform as expected.
Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, June 28, 2010 10:55 AM To: histonet@lists.utsouthwestern.edu; rick.garnh...@memorialhealthsystem.com Subject: SPAM-LOW: Re: [Histonet] Unstained Slides We bake our unstains at 60 degrees C. for 30 minutes prior to filing at RT. There is no set rule for stability. It all depends on fixation, the nature of the protein target, and storage conditions. I've seen absolutely spectacular immunoreactivity on unstained slides stored at RT for 20 years (and longer) and I've seen reduced immunoreactivity in unstained slides stored for as little as 4 weeks. We will always attempt to stain unstained slides when available; however, if the lesion or tumor is negative, and there is no internal control, you better cut fresh sections. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> <rick.garnh...@memorialhealthsystem.com> 6/28/2010 11:54 AM >>> Histoland, How is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet