Re: [Histonet] Giemsa vs Diff Quik
Hi, Have you tried leishman staining? I have an automated protocol for he thermo gemini if you want one? Failing that I do have the manual step protocol and all you need is a jar of leishman powder, methanol and pH6.8 phosphate buffet tablets Stuart Beaver BSc(Hons) Head of Veterinary Histology/Cytology +447568543761 On 6 Sep 2018, at 21:37, Bob Richmond mailto:rsrichm...@gmail.com>> wrote: Joseph A. Esposito at McClain Laboratories on Long Island asks: The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This has caused us to begin to consider the Giemsa stain as an alternative to the Diff Quik. Does anyone have any experience with using both stains and how they compare to each other? Would the Giemsa be a suitable alternative to replace the Diff Quik?<< Diff-Quik® (spelt like that!) is an old registered trademark for a rapid two-step Romanowsky stain. A number of Web sites inform me today that this trade name has been discontinued, I suppose because the name has been so commonly used generically. There have long been a number of generic fast two-step Romanowsky stains available, with a xanthene dye (presumably eosin Y) as solution 1, and a proprietary mixture of thiazine dyes as solution 2. In my experience with at least five of these, they all worked pretty much indistinguishably from brand-name Diff-Quik. There are a number of stains called Giemsa, also Romanowsky stains, but often slower than the "quick" stains. There is probably no advantage to using them for any reason. In my eightieth year, I remember with nostalgia the old Wolbach Giemsa technique for tissue sections. Tissue must be fixed in a dichromate fixative, traditionally Zenker/Helly, sections stained in four successive Giemsa baths, the last one overnight, and differentiated with 10% colophonium rosin in alcohol with microscopic control. (I've actually done this stain myself.) Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Giemsa vs Diff Quik
Joseph A. Esposito at McClain Laboratories on Long Island asks: >>The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This has caused us to begin to consider the Giemsa stain as an alternative to the Diff Quik. Does anyone have any experience with using both stains and how they compare to each other? Would the Giemsa be a suitable alternative to replace the Diff Quik?<< Diff-Quik® (spelt like that!) is an old registered trademark for a rapid two-step Romanowsky stain. A number of Web sites inform me today that this trade name has been discontinued, I suppose because the name has been so commonly used generically. There have long been a number of generic fast two-step Romanowsky stains available, with a xanthene dye (presumably eosin Y) as solution 1, and a proprietary mixture of thiazine dyes as solution 2. In my experience with at least five of these, they all worked pretty much indistinguishably from brand-name Diff-Quik. There are a number of stains called Giemsa, also Romanowsky stains, but often slower than the "quick" stains. There is probably no advantage to using them for any reason. In my eightieth year, I remember with nostalgia the old Wolbach Giemsa technique for tissue sections. Tissue must be fixed in a dichromate fixative, traditionally Zenker/Helly, sections stained in four successive Giemsa baths, the last one overnight, and differentiated with 10% colophonium rosin in alcohol with microscopic control. (I've actually done this stain myself.) Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Giemsa vs Diff Quik
For ordering the Diff-Quik alternative this link works better. It takes you directly to the manufacturer EKI. https://eki-chem.com/product/2295 The labsco link below goes to McKesson. Mike -Original Message- From: Mike Toole via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, September 06, 2018 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Giemsa vs Diff Quik I ordered this a few weeks ago: http://www.labsco.com/items/EKI2295 STAIN BRI KT 16OZ KT 16OZ / 500ML DIFFERENTIAL RAPID BLOOD STAIN SOLUTION KIT, FOR DIFFERENTIAL STAINING OF BLOOD SMEARS, INCLUDES: XANTHENE DYE SOLUTION (A), THIAZINE DYE SOLUTION (B), FIXATIVE SOLUTION (C), 3 X 16OZ HAZMAT Item Id: EKI2295 Supplier Part Number 2295-500ML Supplier Name E K INDUSTRIES, INC. Stock 14 Availability Item in stock. Ships within one business day. Mike -Original Message- From: Laurie Colbert via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, September 06, 2018 10:01 AM To: jose...@mcclainlab.com; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Giemsa vs Diff Quik We use the Diff Quik in place of the Giemsa stain for H. Pylori on gastric bx's. I believe you can use the Diff Quik for FNA's, especially if it is just for a preliminary diagnosis and you will be staining with H or a cytology stain later. We get our Diff Quik from Medical Chemical Corporation. Laurie Redmond -Original Message- From: Joseph Esposito via Histonet To: histonet Sent: Thu, Sep 6, 2018 7:42 am Subject: [Histonet] Giemsa vs Diff Quik The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This has caused us to begin to consider the Giemsa stain as an alternative to the Diff Quik. Does anyone have any experience with using both stains and how they compare to each other? Would the Giemsa be a suitable alternative to replace the Diff Quik? Joseph A. Esposito McClain Laboratories, LLC. (631) 361- 4000 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Giemsa vs Diff Quik
I ordered this a few weeks ago: http://www.labsco.com/items/EKI2295 STAIN BRI KT 16OZ KT 16OZ / 500ML DIFFERENTIAL RAPID BLOOD STAIN SOLUTION KIT, FOR DIFFERENTIAL STAINING OF BLOOD SMEARS, INCLUDES: XANTHENE DYE SOLUTION (A), THIAZINE DYE SOLUTION (B), FIXATIVE SOLUTION (C), 3 X 16OZ HAZMAT Item Id: EKI2295 Supplier Part Number 2295-500ML Supplier Name E K INDUSTRIES, INC. Stock 14 Availability Item in stock. Ships within one business day. Mike -Original Message- From: Laurie Colbert via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, September 06, 2018 10:01 AM To: jose...@mcclainlab.com; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Giemsa vs Diff Quik We use the Diff Quik in place of the Giemsa stain for H. Pylori on gastric bx's. I believe you can use the Diff Quik for FNA's, especially if it is just for a preliminary diagnosis and you will be staining with H or a cytology stain later. We get our Diff Quik from Medical Chemical Corporation. Laurie Redmond -Original Message- From: Joseph Esposito via Histonet To: histonet Sent: Thu, Sep 6, 2018 7:42 am Subject: [Histonet] Giemsa vs Diff Quik The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This has caused us to begin to consider the Giemsa stain as an alternative to the Diff Quik. Does anyone have any experience with using both stains and how they compare to each other? Would the Giemsa be a suitable alternative to replace the Diff Quik? Joseph A. Esposito McClain Laboratories, LLC. (631) 361- 4000 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Giemsa vs Diff Quik
We use the Diff Quik in place of the Giemsa stain for H. Pylori on gastric bx's. I believe you can use the Diff Quik for FNA's, especially if it is just for a preliminary diagnosis and you will be staining with H or a cytology stain later. We get our Diff Quik from Medical Chemical Corporation. Laurie Redmond -Original Message- From: Joseph Esposito via Histonet To: histonet Sent: Thu, Sep 6, 2018 7:42 am Subject: [Histonet] Giemsa vs Diff Quik The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This has caused us to begin to consider the Giemsa stain as an alternative to the Diff Quik. Does anyone have any experience with using both stains and how they compare to each other? Would the Giemsa be a suitable alternative to replace the Diff Quik? Joseph A. Esposito McClain Laboratories, LLC. (631) 361- 4000 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
