Re: [Histonet] PIG processing tissue help

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hello,

we process heart and other biopsies of muscles in Bouin oder Bouin-Hollande 
fixative which both also work fine with IHC and give very brilliant results 
with any kind of trichrome stainings.

With best regards
Michael





> Am 06.09.2017 um 18:22 schrieb Blanca Lopez via Histonet 
> :
> 
> Hello Histonettes,
> I am looking for a better information on what is the best way to treat, fix 
> and process pig heart. This is a very new thing for me because I usually work 
> with mouse and human tissue. Is anybody there that can help me with this 
> please? I will appreciate any feedback event to do perform special stains and 
> IHC for this project,
> Thanks
> 
> 
> 
> UT Southwestern
> 
> 
> Medical Center
> 
> 
> 
> The future of medicine, today.
> 
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MORPHISTO Evolutionsforschung und Anwendung GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

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Re: [Histonet] PIG processing tissue

[E. Wayne johnson via Histonet]

We routinely examine pig heart as part of diagnostic histopathology

and generally we are looking for the lesions of Vitamin E / Selenium 
deficiency.


Vitamin E and organic Se are expensive and often deficient in commercial 
piglet diets.


We simply fix with a formalin based fixative and process routinely to 
paraffin


and stain with our H & E.

-

E. Wayne Johnson

Enable AgTech, Beijing.

"Never do anything mean to a pig.  They'll squeal on you every time."




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