A very interesting question, and I suspect you will receive a wide range of answers. I, too, was taught to cut the first level, then 3 unstained for possible IHC, then second and 3rd level. However, further research has shown that micrometastisis has little effect on patient outcomes so in many labs, the IHC performed on sentinel lymph nodes went out of "fashion". For those nodes that are frozen and show metastatic disease, there is little to gain from additional studies, other than one good H&E for permanent section. For other lymph nodes, we usually start with 3 levels for H&E, 40 microns apart, though I have worked before where the pathologists required 5 levels. It will be interesting to see the varied responses. Thanks for posing this interesting question. Terri
Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 Honesty AccouNtability AgiLity CoLlaboration CoMpassion -----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> Sent: Monday, October 3, 2022 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 227, Issue 2 CAUTION: This email originated from outside Redeemer Health. Do not click links or open attachments unless you recognize the sender and know the content is safe. Contact our IT Support Center at 215-938-3900 with questions. Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Sentinel lymph node microtomy (Samantha Golden) ---------------------------------------------------------------------- Message: 1 Date: Sun, 2 Oct 2022 18:38:34 +0000 (UTC) From: Samantha Golden <samantha.gol...@ymail.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Sentinel lymph node microtomy Message-ID: <2000942797.1958358.1664735914...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 I am certain this question has been asked before, but I could not find a clear, definitive answer; perhaps there isn't one but I'm going to ask anyway... Is there any type of standard for cutting levels on tissue, this example referring to SLN. I was taught to get a representative section for H&E and IHC, go deeper into block for additional sections (50-100 um, using professional judgement), then repeating for the final level(s).? This results in varying, representative levels throughout the block, plus it leaves tissue for additional studies if necessary. However I've realized that not everyone was taught this same way. I want to be certain I am teaching others a correct way, and would love to have something concrete to refer to for guidance.? Thanks for any help! ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 227, Issue 2 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet