Re: [Histonet] Unencased Ameoba Stain
Getting back a little late (been out of state). If this is Acanthamoeba, then we do a PASH and/or a GMS. These are cysts, and show up nicely with both. The cornea can be PAS positive also, so the Acanthamoeba are a darker pink against a lighter pink, while the GMS is gray/black against the green background. It looks a lot like pneumocystis with a GMS, but without the bull's eye, and about 2-3 times larger. But it's the cyst wall that is staining with the PAS and the GMS, as the cyst has a lot of glycogen. But you are asking for unencased amoeba. So that sounds like no cyst. Are you looking for just the trophozoites? I would suggest one of the Giemsa stains, or maybe a Brown and Hopps. A cresyl echt violet (actually cresyl violet acetate, but everyone still calls it by it's old name), would probably also work - I read about it in the NSH Journal of Histotechnology about 20 years ago for the trophozoites of pneumocystis, but I've never tried the stain. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health System Royal Oak, MI 48073 The opinions expressed are mine, and do not represent BHS. -Original Message- From: Joseph Brooks Sent: Wednesday, February 27, 2013 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unencased Ameoba Stain Hello All, One of our Neuropath docs is inquiring about a special stain for unencased ameobas in cornea biopsies. I did a search and Gridley's Method was the best option that appreaded. Is there someone that could either verify this stain will work on this organism or let me know what you stain you are using? Thanks in advance. Matt Brooks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Unencased Ameoba Stain
Either Gridley's method or an ordinary PAS will work very well, due to the high concentration of glycogen in the amoebic cytoplasm. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joseph Brooks Sent: Wednesday, February 27, 2013 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unencased Ameoba Stain Hello All, One of our Neuropath docs is inquiring about a special stain for unencased ameobas in cornea biopsies. I did a search and Gridley's Method was the best option that appreaded. Is there someone that could either verify this stain will work on this organism or let me know what you stain you are using? Thanks in advance. Matt Brooks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unencased Ameoba Stain
The "standard" amoeba stain is Heidenhain's iron hematoxylin. René J. From: Joseph Brooks To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 27, 2013 11:12 AM Subject: [Histonet] Unencased Ameoba Stain Hello All, One of our Neuropath docs is inquiring about a special stain for unencased ameobas in cornea biopsies. I did a search and Gridley's Method was the best option that appreaded. Is there someone that could either verify this stain will work on this organism or let me know what you stain you are using? Thanks in advance. Matt Brooks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
