Re: [Histonet] aqueous mounting medium
Enzyme histochemistry > On Feb 19, 2014, at 6:42 PM, "Lee & Peggy Wenk" wrote: > > to be used for what? > - Lipids - ORO, Sudan black B? > - Immunofluorescence - which dye? > > Different needs, different requirements. > > Peggy A. Wenk, HTL(ASCP)SLS > > -Original Message- > From: Jennifer MacDonald > Sent: Wednesday, February 19, 2014 12:27 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] aqueous mounting medium > > Does anyone have a good "recipe" for aqueous mounting medium? > Thanks, > Jennifer > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] aqueous mounting medium
to be used for what? - Lipids - ORO, Sudan black B? - Immunofluorescence - which dye? Different needs, different requirements. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Jennifer MacDonald Sent: Wednesday, February 19, 2014 12:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] aqueous mounting medium Does anyone have a good "recipe" for aqueous mounting medium? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] aqueous mounting medium
I'm using modified protocol. I'm storing it at 4C. *Aqueous Mounting Medium (glycerol gelatin)* *Kisser's**Kaiser's**Glycerol jelly**Modified*Gelatine10 g40 g65 g5 gDistilled water35 ml210 ml300 ml50 mlGlycerol (glycerin)30 ml250 ml100 ml50 mlPhenol (carbolic acid) 5 ml5 ml Mix gelatin and distilled water. Heat in 50 C oven until gelatin is dissolved (it takes about 30 minutes). Add glycerin and adjust pH to 7.0 using 1N NaOH (to avoid fading of hematoxylin). Store at room temperature. Warm to 37-40 C before mounting. Note: Modified protocol works well. from ihcworld.com On Wed, Feb 19, 2014 at 8:22 PM, Caroline Miller wrote: > I use prolong gold from life tech (formerly molecular probes and now part > of the world dominating thermofisher) without Dapi as I do that separately, > for IF and aquaslip from American master tech for non IF aqueous mounting > > Good luck! > Caroline > > Caroline Miller > Gladstone Institutes > www.gladstoneinstitutes.org > > Tel: 415 7342566 > Cell: 415 2187297 > > > > > > On Feb 19, 2014, at 10:11 AM, Hans B Snyder > wrote: > > > > Hello, > > > > I have one for ORO but not for IF. If someone has one for IF, I would > be interested also. > > > > Thank you > > > > Histologistics > > Hans B Snyder > > 508.308.7800 > > h...@histologistics.com > > > >> On Feb 19, 2014, at 12:27, Jennifer MacDonald > wrote: > >> > >> Does anyone have a good "recipe" for aqueous mounting medium? > >> Thanks, > >> Jennifer > >> ___ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 Asst. Prof. Dr. Mehmet Fatih BOZKURT Manager of Veterinary Diagnostic Laboratory Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16300-16301 vetana...@aku.edu.tr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] aqueous mounting medium
I use prolong gold from life tech (formerly molecular probes and now part of the world dominating thermofisher) without Dapi as I do that separately, for IF and aquaslip from American master tech for non IF aqueous mounting Good luck! Caroline Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 > On Feb 19, 2014, at 10:11 AM, Hans B Snyder wrote: > > Hello, > > I have one for ORO but not for IF. If someone has one for IF, I would be > interested also. > > Thank you > > Histologistics > Hans B Snyder > 508.308.7800 > h...@histologistics.com > >> On Feb 19, 2014, at 12:27, Jennifer MacDonald wrote: >> >> Does anyone have a good "recipe" for aqueous mounting medium? >> Thanks, >> Jennifer >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] aqueous mounting medium
Hello, I have one for ORO but not for IF. If someone has one for IF, I would be interested also. Thank you Histologistics Hans B Snyder 508.308.7800 h...@histologistics.com > On Feb 19, 2014, at 12:27, Jennifer MacDonald wrote: > > Does anyone have a good "recipe" for aqueous mounting medium? > Thanks, > Jennifer > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
