Re: [Histonet] demonstration of asbestos by means of electron microscopy
We showed aspestos fibers with EM by using a formvar coated 200 mesh grids. 1 micro liter sample solution was put on the surface of the formwar coated grid. The grid was dried at room temperature and directly searched with EM. - Orijinal Mesaj - Kimden: Rene J Buesa rjbu...@yahoo.com Kime: histonet@lists.utsouthwestern.edu, Yolanda Davies yolanda.dav...@uct.ac.za Gönderilenler: 22 Kasım Salı 2011 16:28:29 Konu: Re: [Histonet] demonstration of asbestos by means of electron microscopy Many years ago asbestos fibers were demonstrated in hypochlorite digested tissues. The remaining material was filtered through Milllipore filter, washed and the fibers observed with polarized light. The Perl's method will stain no the asbestos fibers (that cannot be stained) but a reactive capsule the tissues secrete around the fiber, so it is an indirect method based in: if there is a tissue reaction to asbestos, they should have been present at a certain moment. As to its demonstration with EM I do not know but I think it will be very difficult to infiltrate the fibers in Epoxi resin to be cut. If you are able to do that: how are you going to identify an extremely thin section of asbestos? I think that you should either try the tissue digestion or give another try at the Perl reaction. René J. --- On Tue, 11/22/11, Yolanda Davies yolanda.dav...@uct.ac.za wrote: From: Yolanda Davies yolanda.dav...@uct.ac.za Subject: [Histonet] demonstration of asbestos by means of electron microscopy To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 22, 2011, 4:14 AM Dear all I am a histotechnologist in forensics, Cape Town, South Africa. I received a request to show asbestos in lung tissue where there is definite interstitial fibrosis, but the presence of asbestos is not clear. Is it possible to reveal asbestos by means of electron microscopy? Usually asbestos is demonstrated using the Perl's Prussian blue technique, but most times they are elusive. Could it be because of the sampling site or simply the nature of the asbestos? Thank you in advance Yolanda Davies Department of Forensic Medicine and Toxicology Falmouth building Anzio Road Observatory Cape Town South Africa ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Emin Oztas, MD. PhD. Gulhane Military Medical Academy Department of Medical Histology and Embryology Ankara, 06018, Turkey eminoz...@gata.edu.tr +90 312 3043536 +90 532 5215952 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] demonstration of asbestos by means of electron microscopy
The StainsFile page has some techniques, using polarizing microscopes. http://stainsfile.info/StainsFile/stain/pigment/asbestos.htm Asbestos are usually very small fibers, and will not show up in every section. Cutting thicker sections sometimes helps. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -Original Message- From: Yolanda Davies Sent: Tuesday, November 22, 2011 4:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] demonstration of asbestos by means of electron microscopy Dear all I am a histotechnologist in forensics, Cape Town, South Africa. I received a request to show asbestos in lung tissue where there is definite interstitial fibrosis, but the presence of asbestos is not clear. Is it possible to reveal asbestos by means of electron microscopy? Usually asbestos is demonstrated using the Perl's Prussian blue technique, but most times they are elusive. Could it be because of the sampling site or simply the nature of the asbestos? Thank you in advance Yolanda Davies Department of Forensic Medicine and Toxicology Falmouth building Anzio Road Observatory Cape Town South Africa ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] demonstration of asbestos by means of electron microscopy
Yolanda, Yes it is, TEM is often used for asbestos detection in lung tissue to identify chrysotile and/or crocidolite (mesothelioma-associated) asbestos fibers. There are a lot of publications out there... Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yolanda Davies Sent: Tuesday, November 22, 2011 4:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] demonstration of asbestos by means of electron microscopy Dear all I am a histotechnologist in forensics, Cape Town, South Africa. I received a request to show asbestos in lung tissue where there is definite interstitial fibrosis, but the presence of asbestos is not clear. Is it possible to reveal asbestos by means of electron microscopy? Usually asbestos is demonstrated using the Perl's Prussian blue technique, but most times they are elusive. Could it be because of the sampling site or simply the nature of the asbestos? Thank you in advance Yolanda Davies Department of Forensic Medicine and Toxicology Falmouth building Anzio Road Observatory Cape Town South Africa ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] demonstration of asbestos by means of electron microscopy
Many years ago asbestos fibers were demonstrated in hypochlorite digested tissues. The remaining material was filtered through Milllipore filter, washed and the fibers observed with polarized light. The Perl's method will stain no the asbestos fibers (that cannot be stained) but a reactive capsule the tissues secrete around the fiber, so it is an indirect method based in: if there is a tissue reaction to asbestos, they should have been present at a certain moment. As to its demonstration with EM I do not know but I think it will be very difficult to infiltrate the fibers in Epoxi resin to be cut. If you are able to do that: how are you going to identify an extremely thin section of asbestos? I think that you should either try the tissue digestion or give another try at the Perl reaction. René J. --- On Tue, 11/22/11, Yolanda Davies yolanda.dav...@uct.ac.za wrote: From: Yolanda Davies yolanda.dav...@uct.ac.za Subject: [Histonet] demonstration of asbestos by means of electron microscopy To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 22, 2011, 4:14 AM Dear all I am a histotechnologist in forensics, Cape Town, South Africa. I received a request to show asbestos in lung tissue where there is definite interstitial fibrosis, but the presence of asbestos is not clear. Is it possible to reveal asbestos by means of electron microscopy? Usually asbestos is demonstrated using the Perl's Prussian blue technique, but most times they are elusive. Could it be because of the sampling site or simply the nature of the asbestos? Thank you in advance Yolanda Davies Department of Forensic Medicine and Toxicology Falmouth building Anzio Road Observatory Cape Town South Africa ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
