Re: [Histonet] microtome safety
Every tech in my facility uses something different. Some use fingers, some use forceps (usually curved), one uses a teasing needle and one uses a paint brush. It's up to the individual's technique but we do try to discourage using one's fingers too close to the blade. Hope this helps. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue, March 8, 2011 8:20:16 AM Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome safety
Same here. One tech keeps her index fingernail extra long for this purpose. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Sheila Haas Sent: Tuesday, March 08, 2011 8:26 AM To: Bartlett, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] microtome safety Every tech in my facility uses something different. Some use fingers, some use forceps (usually curved), one uses a teasing needle and one uses a paint brush. It's up to the individual's technique but we do try to discourage using one's fingers too close to the blade. Hope this helps. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue, March 8, 2011 8:20:16 AM Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome safety
I've always used my fingers but some others in my lab use forceps. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microtome safety
I always found better and used a wet camel's hair pencil. It provides the most gentle pull on the sections. René J. --- On Tue, 3/8/11, Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov Subject: [Histonet] microtome safety To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, March 8, 2011, 8:20 AM Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome safety
Jeanine, I use forceps with the tips bent at a 90 degree angle for reaching under the sections to remove air bubbles. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 08, 2011 6:54 AM To: histonet@lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett Subject: Re: [Histonet] microtome safety I always found better and used a wet camel's hair pencil. It provides the most gentle pull on the sections. René J. --- On Tue, 3/8/11, Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov Subject: [Histonet] microtome safety To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, March 8, 2011, 8:20 AM Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome safety
Actually I need to qualify my answer, I use my fingers to grab end of the ribbon the farest from the blade and curved forceps near the blade so I use both finger and forceps at the same time. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: Patsy Ruegg [mailto:pru...@ihctech.net] Sent: Tuesday, March 08, 2011 7:16 AM To: 'Rene J Buesa'; 'histonet@lists.utsouthwestern.edu'; 'Jeanine (CDC/OID/NCEZID)Bartlett' Subject: RE: [Histonet] microtome safety Jeanine, I use forceps with the tips bent at a 90 degree angle for reaching under the sections to remove air bubbles. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 08, 2011 6:54 AM To: histonet@lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett Subject: Re: [Histonet] microtome safety I always found better and used a wet camel's hair pencil. It provides the most gentle pull on the sections. René J. --- On Tue, 3/8/11, Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov Subject: [Histonet] microtome safety To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, March 8, 2011, 8:20 AM Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome safety
I use forceps, the thinner the tips, the better. I've had people in here who use brushes, too. I can't use my fingers, the ribbons always stick. Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 Same here. One tech keeps her index fingernail extra long for this purpose. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Sheila Haas Sent: Tuesday, March 08, 2011 8:26 AM To: Bartlett, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] microtome safety Every tech in my facility uses something different. Some use fingers, some use forceps (usually curved), one uses a teasing needle and one uses a paint brush. It's up to the individual's technique but we do try to discourage using one's fingers too close to the blade. Hope this helps.  Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc.  From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue, March 8, 2011 8:20:16 AM Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome safety
I use the end (without bristles) of a paintbrush Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome safety
Just regular forceps. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microtome safety
I use curved forceps to hold the ribbon and a paint brush to release it from the knife edge. The curved forcepts help to separate the sections and remove air bubbles. Jennifer MacDonald Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov Sent by: histonet-boun...@lists.utsouthwestern.edu 03/08/2011 05:25 AM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microtome safety
We use fingers...I am not aware of any kind of forceps. But along with you i am also curious to know if any thing else is available. Amita From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 08/03/11 06:52 PM Subject: [Histonet] microtome safety Sent by: histonet-boun...@lists.utsouthwestern.edu Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
