Re: [OGD] Viruses, again
I have today received no less than five separate virus attempts from rforchid labeled things like funny. One assumes, once again, that the source is this list. __ Oliver Sparrow +44 (0)20 7736 9716 www.chforum.org ___ the OrchidGuide Digest (OGD) orchids@orchidguide.com http://orchidguide.com/mailman/listinfo/orchids_orchidguide.com
Re: [OGD] viruses again
Keith, I have performed and developed ELISAs in my line of work as a Research Assistant in Molecular Biology and Immunology. Each assay, done in panels, requires a row, or serial dilution, of standards to be performed with it. The dilutions should then match up with results in a stepwise fashion when plotted on a graph (standard curve). Test sample results are then plotted along that line to give the amount of agent being searched for present. Getting lazy and using one standard curve for several tests (on different assay plates) assures that plate to plate variation will give inaccurate results, especially if done on different days with different reagent stocks used.Ways in which samples are prepared for assays can also obscure results and all samples should be done in at least duplicate, preferably triplicate or quadruplicate to help screen out false positives, but this is not assured. Bonaventure --Message: 4Date: Wed, 30 Aug 2006 14:56:50 +From: "K Barrett" [EMAIL PROTECTED]Subject: Re: [OGD] viruses againTo: orchids@orchidguide.comMessage-ID: [EMAIL PROTECTED]Content-Type: text/plain; charset="iso-8859-1"Thanks for doing that Cynthia. Open question: Does anyone have any other experience with the ELISA test (even in another line of work) so we can judge as to how correct it is? Like the rates of false negatives/false positives? K BarrettN Calif, USA ___ the OrchidGuide Digest (OGD) orchids@orchidguide.com http://orchidguide.com/mailman/listinfo/orchids_orchidguide.com
Re: [OGD] Viruses again
Thanks to Terry Glancy and Robert Bedard for their information in the last digest. Its nice to know how virulent these viruses are.I'm glad someone is going forward with PCR protocols in virus testing. I imagine the stumbling block to expanding this to other viruses is getting the infrastructure in place. I'm always the entrepreneur, and wonder if this could become a cottage industry forretired science hobbyists, or forward thinking grad students. Heck, better yet, an underpaid tech with delusions of grandeur.*G*.(Nah, its probably prohibited in Genetech's employment contracts) Maybe there'd be some grant money out there for research into identification isolation of other plant viruses. Anybody know J Craig Ventner personally? Maybe he'd taketime away from his current project to elucidate the DNA sequences for all 40 plant viruses. http://www.sorcerer2expedition.org/version1/HTML/main.htm "I got vision and the rest of the world's wearing bifocals" Butch Cassidy Barrett N Calif, USASearch from any Web page with powerful protection. Get the FREE Windows Live Toolbar Today! Try it now! ___ the OrchidGuide Digest (OGD) orchids@orchidguide.com http://orchidguide.com/mailman/listinfo/orchids_orchidguide.com
Re: [OGD] viruses again
Thanks for doing that Cynthia. Open question: Does anyone have any other experience with the ELISA test (even in another line of work) so we can judge as to how correct it is? Like the rates of false negatives/false positives? K Barrett N Calif, USA Ihaveupdatedmy'VirusinOrchids'pbasesiteandincludedthetypeof virus. http://www.pbase.com/schnitz/virus_in_orchids InoticethatIdon'thaveanyleafpicturesforORSVonly.CMVapearsto occurabout4timesasofteninsinglyinfectedplants.Iwilllookover theremainingvirusedplantswhenIhavetimetoseeifthereareanygood symptomsinORSVinfectedplantstotakepicturesof.Iambeginningto suspectthattheORSVdataisnotareliableastheCMVdata.Cynthia, Prescott,AZCheck the weather nationwide with MSN Search Try it now! ___ the OrchidGuide Digest (OGD) orchids@orchidguide.com http://orchidguide.com/mailman/listinfo/orchids_orchidguide.com
