[R] How should I do GO enrichment of differential expressed miRNA?

2014-08-29 Thread my1stbox
Hi all,
First, I carried out GO enrichment to predicted/validated target genes of those 
miRNA using GOstats package. Then I find myself in a dead end. So what is the 
good practice? Is it possible to directly do GO enrichment to miRNAs? Are they 
included in GO database?
Regards,
Allen
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[R] What would a typical miRNA microarray analysis workflow look like?

2014-08-26 Thread my1stbox
Hi all,
What would a typical miRNA microarray analysis workflow look like? Say just a 
test group of 5 replicates from bladder cancer tumor and corresponding control 
group from normal tissue of the same patients. What could I do to make the 
analysis seems more sophisticated. I have done differential expression 
analysis, target gene prediction, and GO, pathway enrichments of target genes. 
What else could I do? It would be better if you can specify some packages or 
codes.
Regards,
Allen

PS: Is paired (t)-test necessery? I found that DE gene number from paired 
t-test is about half of that from normal limma functions( which I don't know 
how to do paired test if it could).

2014-08-26
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Re: [R] How to do t.test to rows of a dataframe using apply family function?

2014-08-26 Thread my1stbox
Thank you so much!
Why do do.call(rbind,lapply(lll,rbind)) and rbind(lapply(lll,rbind)) act so 
differently? What is the tricky part of that?
 do.call(rbind,lapply(lll,rbind))
statistic parameter p.value conf.int estimate null.value alternative
[1,] 2.775282 37.99977 0.008509272 Numeric,2 Numeric,2 0 two.sided
[2,] -1.498133 37.31294 0.1425116 Numeric,2 Numeric,2 0 two.sided
method data.name 
[1,] Welch Two Sample t-test rnorm(20) and rnorm(20)
[2,] Welch Two Sample t-test rnorm(20) and rnorm(20)
 rbind(lapply(lll,rbind))
[,1] [,2] 
[1,] List,9 List,9

2014-08-26 






发件人:PIKAL Petr petr.pi...@precheza.cz
发送时间:2014-08-26 17:58
主题:RE: RE: [R] How to do t.test to rows of a dataframe using apply family 
function?
收件人:my1stboxmy1st...@163.com
抄送:R Helpr-help@r-project.org

Hi
Please reply also to rhelp, maybe others can offer better answer.
Well, are you aware that by unlist you get all results as character values and 
not numbers?
Personally I would use lapply and do.call on final result to get it as data 
frame.
Here is example
Some model list data
lll-vector(list,length=2)
lll
[[1]]
NULL
[[2]]
NULL
lll[[1]]-t.test(rnorm(20), rnorm(20))
lll[[2]]-t.test(rnorm(20), rnorm(20))
str(unlist(lll))
Named chr [1:22] 0.0091599930484716 37.9972278626102 ...
- attr(*, names)= chr [1:22] statistic.t parameter.df p.value 
conf.int1 ...
do.call(rbind, lapply(lll, rbind))
 statistic   parameter p.value   conf.int  estimate  null.value alternative
[1,] 0.009159993 37.99723  0.9927394 Numeric,2 Numeric,2 0  two.sided
[2,] 0.1256267   32.93057  0.9007913 Numeric,2 Numeric,2 0  two.sided
 methoddata.name
[1,] Welch Two Sample t-test rnorm(20) and rnorm(20)
[2,] Welch Two Sample t-test rnorm(20) and rnorm(20)
Regards
Petr

From: my1stbox [mailto:my1st...@163.com] 
Sent: Tuesday, August 26, 2014 10:44 AM
To: PIKAL Petr
Subject: Re: RE: [R] How to do t.test to rows of a dataframe using apply family 
function?

Hi Petr,
Thank you! I use unlist() and rbind() because I want the result to be in the 
form of a matrix.
Regards
Allen

2014-08-26 







发件人:PIKAL Petr petr.pi...@precheza.cz
发送时间:2014-08-26 14:33
主题:RE: [R] How to do t.test to rows of a dataframe using apply family function?
收件人:my1stboxmy1st...@163.com,R Helpr-help@r-project.org
抄送:

Hi 

If your function works for you why to bother with apply? If it does not give 
you required results, please post some data and show us what is expected. 

I would remove unlist from your function and declare list for storing data, 
which seems to me more natural for storing results. 

t.test.list=function(df,paired){ 
   lll-vector(list, length=nrow(df)) 
   for(i in 1:nrow(df)){ 

 lll[[i]]=t.test(df[i,grp1],df[i,grp2],paired=paired) 
   } 
  names(lll)=rownames(df) 
   lll 
 } 

Regards 
Petr 


 -Original Message- 
 From: r-help-boun...@r-project.org [mailto:r-help-bounces@r- 
 project.org] On Behalf Of my1stbox 
 Sent: Tuesday, August 26, 2014 3:26 AM 
 To: R Help 
 Subject: [R] How to do t.test to rows of a dataframe using apply family 
 function? 
 
 Hi All, 
 How to do t.test  to rows (with two levels, or in other words, groups 
 of samples) of a dataframe using apply family function? I have done 
 that using function and loops. And my overall purpose is to calculate 
 DE genes from two groups of miRNA microarray samples. And I know limma 
 can do that, but it seems limma doesn't support paired t-test. 
 
 t.test.df=function(df,paired){ 
   df.t=c() 
   for(i in 1:nrow(df)){ 
 
 df.t=rbind(df.t,unlist(t.test(df[i,grp1],df[i,grp2],paired=paired))) 
   } 
   rownames(df.t)=rownames(df) 
   df.t 
 } 
 
 Bests! 
 Allen Chiu 
 
 2014-08-26 
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[R] How to do t.test to rows of a dataframe using apply family function?

2014-08-25 Thread my1stbox
Hi All,
How to do t.test  to rows (with two levels, or in other words, groups of 
samples) of a dataframe using apply family function? I have done that using 
function and loops. And my overall purpose is to calculate DE genes from two 
groups of miRNA microarray samples. And I know limma can do that, but it seems 
limma doesn't support paired t-test.

t.test.df=function(df,paired){
  df.t=c()
  for(i in 1:nrow(df)){
df.t=rbind(df.t,unlist(t.test(df[i,grp1],df[i,grp2],paired=paired)))
  }
  rownames(df.t)=rownames(df)
  df.t
}

Bests!
Allen Chiu

2014-08-26
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and provide commented, minimal, self-contained, reproducible code.


[R] What is RVM t test? Is it possible to use it in R?

2014-08-25 Thread my1stbox
Hi All,
What is RVM t test? Is it possible to use it in R? And How?
Bests,
Allen Chiu

2014-08-26
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R-help@r-project.org mailing list
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PLEASE do read the posting guide http://www.R-project.org/posting-guide.html
and provide commented, minimal, self-contained, reproducible code.