Hi all,
What would a typical miRNA microarray analysis workflow look like? Say just a
test group of 5 replicates from bladder cancer tumor and corresponding control
group from normal tissue of the same patients. What could I do to make the
analysis seems more sophisticated. I have done differential expression
analysis, target gene prediction, and GO, pathway enrichments of target genes.
What else could I do? It would be better if you can specify some packages or
codes.
Regards,
Allen
PS: Is paired (t)-test necessery? I found that DE gene number from paired
t-test is about half of that from normal limma functions( which I don't know
how to do paired test if it could).
2014-08-26
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