Re: [spctools-discuss] Tandem Analysis

['Luis Mendoza' via spctools-discuss]

Hello Priti,
You can specify non-enzymatic cleavage by setting the *protein, cleavage
site* parameter to *[X]|[X]* .

More info at the X!Tandem site:
https://www.thegpm.org/TANDEM/api/pcs.html
https://www.thegpm.org/TANDEM/api/pcsemi.html

Hope this helps,
--Luis


On Wed, Aug 3, 2022 at 10:59 PM Priti Panchal 
wrote:

> Hello
> I am trying to do tandem analysis. I am uncertain whether we can perform a
> tandem analysis for the lc-ms/ms data of non enzymatically digested sample
> since in the tandem parameter file there is not any option available for
> "no cleavage" or "no cut" as found in comet parameters. In tandem, there is
> "semi tryptic" digestion option only.
> If we can perform the analysis in tandem please suggest how to edit the
> parameter file to perform analysis of non digested data.
> Thank you
> Priti
>
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>

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[spctools-discuss] Tandem Analysis

[Priti Panchal]

Hello
I am trying to do tandem analysis. I am uncertain whether we can perform a 
tandem analysis for the lc-ms/ms data of non enzymatically digested sample 
since in the tandem parameter file there is not any option available for 
"no cleavage" or "no cut" as found in comet parameters. In tandem, there is 
"semi tryptic" digestion option only. 
If we can perform the analysis in tandem please suggest how to edit the 
parameter file to perform analysis of non digested data. 
Thank you
Priti 

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