[aroma.affymetrix] Re: Using GenomeGraphs with FIRMA (Was: FIRMA score)

2009-03-06 Thread Frank
A note to my own post above: > psCsv <- read.table(HuEx.small.csv,skip=1,sep=" ",comment.char="") > colnames(psCsv) <- c("probeset_id","start", "stop", "probe_count") If you are reading more columns in (like "strand") which are characters, you should keep in mind, that R usually treats them as

[aroma.affymetrix] Re: Using GenomeGraphs with FIRMA (Was: FIRMA score)

2009-03-04 Thread Mark Robinson
Hi Frank. Comments below. On 05/03/2009, at 12:34 PM, Frank Ziegner wrote: > Hi Mark, > > I have some questions regarding you plot and some more general > questions regarding probesets/exons. > > I assume your plot shows the WNK1 (ENSG0060237) gene. > > When doing: > u<-which(getUnitNa

[aroma.affymetrix] Re: Using GenomeGraphs with FIRMA (Was: FIRMA score)

2009-03-04 Thread Frank
Hi everybody, based on Mark's hints I wrote some code to plot FirmaScores with GenomeGraphs. Mark's way of extracting the data (d <-log2(extractMatrix (cs,cells=ind,verbose=verbose)) ) is very elegant and effective. But somehow I didn't manage to pull the firma scores data off in the same way. Th

[aroma.affymetrix] Re: Using GenomeGraphs with FIRMA (Was: FIRMA score)

2009-03-04 Thread Frank
Hi Mark, thanks for your help. The awk-hint is wonderful. The annotation file is MUCH smaller and better manageable in R. I want to give some advice for Windows-Users: - get the UnxUtils (update your PATH variable to use the commands system-wide) - the awk command is called gawk in UnxUtils - on

[aroma.affymetrix] Re: Using GenomeGraphs with FIRMA (Was: FIRMA score)

2009-03-03 Thread Mark Robinson
Hi Frank. A very good question and unfortunately, I haven't had time to put a vignette together. I have given a working example below that will at least to get you started. On 03/03/2009, at 9:34 AM, Mark Robinson wrote: > [this was sent to me offline. i've created a new thread and will t