> On the handling of atomic anisotropic displacement
> parameters
> R. W. Grosse-Kunstleve* and P. D. Adams
> J. Appl. Cryst. (2002). 35, 477-480
>
> to look up the above relationship. In the paper it is mentioned that
>
> |Ucart - (lambda)I|=0
>
> is solved using Cardan's formula.
After the paper
Hi Mary,
not sure if you received this suggestion already.
Despite the low resolution diffraction 4-5 A as you report, try mounting
smaller crystals and see how well they perform. Very often our crystals
had a better freezing experience when they were small, even if
sufficient cryo protectant
CCP4ers:
What is the correct reference for Phil Evans Program POINTLESS?
Thanks for your help!
Rebecca
On Tuesday 10 July 2007 09:16, Mary Fitzgerald wrote:
> If that doesn't work maybe, I'll try seeding at lower MPD
> concentrations or pressure freezing.
>
> Thanks again,
> Mary
With such a high MPD condition I would probably first try micro-seeding at
25-35% MPD before doing anything else. It so
Wow, thanks. I'm going to try to answer most of the questions I've
received in one message as I'm overwhelmed by the quick multitude of
responses.
As I stated earlier, I haven't collected any room temperature data,
yet. So, I don't know the unfrozen mosaicity. It is very possible
that the cry
Hi Mary,
What final percent MPD do you have prior to flashcooling? Karolin Luger (and
perhaps others) found
that the final percent MPD had a significant effect on crystal mosaicity and
final diffraction
limit for nucleosome crystals.
For example, if the crystals are left in mother liquor con
Here's a method that has had some success in reducing mosaicity, either
with or without cryoprotectant:
Chae Un Kim, Raphael Kapfer and Sol M. Gruner (2005), High Pressure
Cooling of Protein Crystals without Cryoprotectants, ActaCryst. D61,
881-890
This method may be available at a synchrotron ne
What is the mosaicity of the unfrozen crystal?
Jim
-Original Message-
From: Mary Fitzgerald <[EMAIL PROTECTED]>
Date: Mon, 9 Jul 2007 18:05:10
To:CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Help with reducing crystal mosaicity
Help please!
I'm looking for some new ideas. I have cr
Help please!
I'm looking for some new ideas. I have crystals that come out of a
sitting drop with a mixture of sodium cacodylate at pH 6.5, magnesium
acetate and MPD for the well solution. The MPD concentration is
sufficient to act as a cryoprotectant. Currently, I directly freeze
these crysta
Dear all,
I'm wondering anyone can recommend some programs or servers to evaluate
protein-protein contacts. I'm currently have a homogeneous tetramer.
However, the protein exists as a dimer in solution. I'm thinking where to
cut the line to generate a dimer from the available structure.
Postdoctoral Positions -- Gaudet Lab -- Harvard University
Postdoctoral positions are available in the Gaudet laboratory
within the Department of Molecular and Cellular Biology at Harvard
University. We use a combination of X-ray crystallography, biochemistry and
functional assays
Hello all,
I'm working on a protein-DNA complex. My protein is a trimer and the crystal has 3 trimers in an AU. I used a pdb file including three subunits as a search model when I did molecular replacement in CCP4. I did a rigid body refinement in Recfmac the R-factor and R-free did not go d
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Hello Fred;
You might be interested in looking at http://xtal.nki.nl/Depot , and
more precisely at http://xtal.nki.nl/cgi-bin/WebObjects/Depot.woa/wa/
tools#qualifiers
You'll see that indeed you can query the DB upon specific criteria
(named s
Hi Fred,
Were I you I would take a look at the archive from the JCSG:
http://www.jcsg.org
Most of the structures are around 50% solvent but I would guess that
there are a few which are much higher... I am sure I have come across
one or two... The advantage of this source is that almost all of th
Dear CCP4BB subscribers,
I am looking for some data to run some tests of methods.
I am interested in the treatment of solvent regions, first
during structure solution by experimental methods (MIRAS, MIR,
MAD, SIRAS etc).
For these tests, I need to access experimental data (Fobs,
experimental pha
Dear all,
I am currently refining the structure of a protein-DNA complex using Refmac.
However, one DNA strand of the dsDNA contains one unusual nucleotide, namely
2`-deoxyuridine ("Ud") which is not defined in the standard Refmac library.
How can I tell Refmac to refine the modified nucleotide "
We would like to announce the Practical Course on Training in methods
for Macromolecular Crystallography M2M-7: From Measurement to Model. The
course will take place at the EMBL Hamburg Outstation on the DESY
synchrotron site in Hamburg on:
Wednesday November 21st - Wednesday November 28th, 20
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