Hi Eleanor and others:
This is a little too late to follow up on this thread. But here is a
similar question concerning MR and self-rotation.
Without going into the translational part of MR, is it possible to tell
the orientation of a 2-fold axis directly from the cross-rotation peaks?
e.g. I ha
Hi all,
thanks a lot for all the responses!
As anticipated, the solution is rather simple - the origin of the beam:
denzo refines to: x=82.6 y=80.3
mosflm reads from header: x=80.0 y=84.0 - bad indexing etc
mosflm works with: x=82.6 y=80.3
No swapping of x and y between denzo and mosflm in this cas
Oh, there is do much you can do!
Lots of alternatives:
1. Decrease or increase the protein concentration (with a corresponding
increase or decrease of precipitant.
2. Use a temperature gradient.
3. Set up in a capillary with liquid-liquid diffusion
4. SEED!
#3 or #4 are your best bets, probably.
Dear all
I am trying to crystallize a 7kDa protein using MPD as a precipitant at
16C.I have got small florets at 55-65% of MPD in 3-4 days.The problem is
that the drop is precipitating in one day only and the crystals are coming
with precipitation.I have added 5% glycerol and 100mM of Nacl as an ad
Hi,
I am looking for information on techniques to do dark current/background
correction on CCD images.
I have found some information of the nonius website
(http://www.nonius.nl/KappaCCD/manuals/collect/inside/dark.html)
but I was hoping to find more. For instance, I seem to remember a
technique
In Coot - turn on the "Cell & Symmetry..." option (in the Draw menu) for
your molecule, in order to visualise the other
molecules within your crystal.
Then use "Save Symmetry Coordinates..." (in the File menu).
Click on the isolated bit of your structure that is closest to the rest
of your molecul
Something else you need to be aware of when using WASP, even if the symmetry
contact problem isn't an issue, e.g. as a work-around for that you could
generate a PDB file containing a complete unit cell with a small border of say
3.5 Ang around it - I've no idea if the server does that for you.
Hi
I'd guess from the input file that the beam centre may be defined in a
different reference frame to that which Mosflm expects in the image
header; other possibilities are that the distance, wavelength, or
something else (!) is wrong. Indexing is critically dependent on having
the beam cent
