[ccp4bb] problems of running mosflm on the MAC

2007-12-20 Thread michael nelson
Hi,folks, I got a brand new Mac Intel. I download the pre-built mosflm 7.0.1 from Harry's website.But I got the following error when I try to execute it, dyld: Library not loaded: /usr/X11R6/lib/libXt.6.dylib Referenced from: /Users/mnelson/downloads/./ipmosflm_osx_intel Reason: image not found

Re: [ccp4bb] Question about protein expression (not CCP4 related)

2007-12-20 Thread Artem Evdokimov
1) Yes. They work "OK" - but I prefer to use dedicated vectors mostly for aesthetic reasons, since the TriEx series are kind of bloated (for a good reason). The expression levels are usually alright. So, in conclusion - if you're trying 100 genes in all kinds of systems you could use TriEx (or Gate

Re: [ccp4bb] Question about protein expression (not CCP4 related)

2007-12-20 Thread Dima Klenchin
1. I was wondering if anyone used the "Multisystem Expression" vectors from Novagen and if so whats your opinion about these vectors? These are the pTriEx series of vectors that can be used for protein expression in E.coli, baculovirus and mammalian cells using the same construct. Since most

[ccp4bb] Post Dr. Position in Taipei

2007-12-20 Thread 袁小琀 Hanna Yuan
Post Doctoral Fellow Academia Sinica, Taiwan Department: Institute of Molecular Biology Location: Taipei, Taiwan Start Date: ASAP Duration: 2 year initially Description: A postdoctoral position is available in the Institute of Molecular Biology at Academia Sinica, Taipei, Taiwan, ROC

[ccp4bb] Question about protein expression (not CCP4 related)

2007-12-20 Thread Anirban Adhikari
Hello, Sorry about the non CCP4 related question. I have few questions regarding protein expression: 1. I was wondering if anyone used the "Multisystem Expression" vectors from Novagen and if so whats your opinion about these vectors? These are the pTriEx series of vectors that can be used fo

Re: [ccp4bb] SHELEX .pdb and .res output and MLPHARE HA file

2007-12-20 Thread George M. Sheldrick
In this case I would recommend searching for more Se atoms, e.g. FIND 6 or 8 for SHELXD. If you then input the results into SHELXE (or are using these programs via hkl2map) you do not need use MLPHARE as well. George Prof. George M. Sheldrick FRS Dept. Structural Chemistry, University of Goe

[ccp4bb] Lead Web Architect, PDB at UCSD

2007-12-20 Thread Peter Rose
The following position is available at the RCSB Protein Data Bank at UCSD: The RCSB PDB at the University of California, San Diego has a position open for a Lead Web Architect. The incumbent will function as a lead architect and Enterprise Java web developer of the RCSB Protein Data Bank (PDB)

[ccp4bb] Senior Scientist/Scientific Software Developer, PDB at UCSD

2007-12-20 Thread Peter Rose
The following position is available at the RCSB Protein Data Bank at UCSD: Function as a senior scientist and scientific software developer for the RCSB Protein Data Bank (PDB; http://www.pdb.org) at the University of California San Diego (UCSD). Work closely and collab

Re: [ccp4bb] Coot 0.4 on Mac OS X 10.4

2007-12-20 Thread Mischa Machius
On Dec 20, 2007, at 10:26 AM, Juergen Bosch wrote: Wrong board, I see it as one unified world of structural biology. I have stopped distinguishing between the boards and preceding messages with "Off topic..." Pretty much anyone who is subscribed to cootbb is also subscribed ccp4bb. An

Re: [ccp4bb] Coot 0.4 on Mac OS X 10.4

2007-12-20 Thread Juergen Bosch
Wrong board, here's what Bill posted to the cootbb yesterday: Hope that solves your problem, Juergen forwarded email: Hi Folks: In response to the numerous death threats, I've tried to make a stand- alone coot for OS X 10.5 intel. http://tinyurl.com/24mchk It might work on 10.4 intel as

[ccp4bb] Coot 0.4 on Mac OS X 10.4

2007-12-20 Thread Mischa Machius
Y'all - I was wondering if anyone had a precompiled, stand-alone binary (Universal or Intel) of coot 0.4 for Mac OS X 10.4 that is not dependent on Fink (or anything else, for that matter)? Thanks so much. Best - MM

[ccp4bb] Postdoctoral Research position, University of Cambridge

2007-12-20 Thread Peter leadlay
Dear All This current vacancy may be of interest to final year PhD or recently postdoctoral researchers. Best wishes Peter Leadlay Professor Peter Leadlay, University of Cambridge, Department of Biochemistry, Cambridge CB2 1GA, UK. HERCHEL SMITH POSTDOC

Re: [ccp4bb] SHELEX .pdb and .res output and MLPHARE HA file

2007-12-20 Thread Eleanor Dodson
You probably dont need to refine the positions but i guess there is no harm in it.. You do need to refine the ano occupancy which reflects the f" values. Your sites 2 and 4 are quite close so the MSE might be disordered? It doesnt really matter what you call the atom type since nothing is on th

[ccp4bb] SHELEX .pdb and .res output and MLPHARE HA file

2007-12-20 Thread Partha Chakrabarti
Hiya, I used shelex C/D/E on a SAD data, and wanted to refile the positions in MLPHARE before going for AddSOLVE. Confused with the output of two shelex files. shelx1.pdb file says CRYST1 50.050 65.520 105.180 90.00 90.00 90.00 SCALE1 0.019980 0.00 0.000.0 SCA