Dear All,
I installed ccp4 6.0.2 on my fedora8 platform. It
seems I need one extra file named "setpaths.sh", which I can not
find in
$CCP4/lib/cctbx/cctbx_build/� &�
${CCP4}/src/phaser/phaser-${phaser_version}/build/${phaser_mtype}/
Where can I get such a file?
Thanks in advance.
Sincerely,
Hi Li,
setpaths.sh (and variants for other shells) are generated during the cctbx
build process (with ccp4-6.0.2, this might happen only if you're installing
phaser).
If you're doing a source install from both ccp4-6.0.2.tar.gz and
phaser-1.3.3-cctbx-src.tar.gz, and have extracted both of th
In general I think they should be ok. Some componenents may slowly oxidize or
otherwise deteriorate, but as long as there is nothing growing in the
solutions, you should be ok. If you experience irreproducibility when you try
to follow up on a successful condition, it is good to keep in mind th
Dear CCP4 users,
I'm sorry for non-ccp4 question. My lab is using protein
crystalization screening from Hampton and Emerald. However I noticed
the Expires Date was 05/2002. That means we are using the too old
chemical.
Could I ask your experience about this problem? Should I need to
remove these ex
This is a tough question. Certainly, in an ideal world (and also in the
world that e.g. Hampton or Emerald salespeople would like us to live in) you
should replace expired reagents. The reality of course is that it's
sometimes quite costly to do so. It is also expensive (in terms of time and
effort
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Dear all,
I was about to write something quite related, but as the topic has
just started ... I had better add my comments.
I am just establishing a crystallization facility, but currently in
my lab proteins to crystallize will be a rare opportunity (hopefully to
increase the number duri
We used to freeze screens in 96-well deep blocks and plates - with no
apparent issues :-)
Some stuff crystallizes out but usually goes right back into solution.
Artem
_
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of iulek
Sent: Thursday, December 27, 2007 7:39
The problem with old solutions is not their age as much as they might be
very hard to reproduce, as you won't exactly know what you have. (for
all the reasons that have been mentioned: evapouration, degradation, pH
shifts, mould growth, contamination from people using the wrong pipette
tip in a di
