Re: [ccp4bb] ideal bond length and bong angel

2008-01-10 Thread Eleanor Dodson
Please do NOT use the BB for abuse. Eleanor Gerard DVD Kleywegt wrote: Be care, you insolence might get someone sent disappear. is the chinese secret service editing my incoming mail already, or are you merely using a microsoft grammar checker? --dvd

Re: [ccp4bb] bond lengths, angles, ideality and refinements

2008-01-10 Thread Dirk Kostrewa
Am 09.01.2008 um 20:48 schrieb Anastassis Perrakis: snip I actually think that inaccurate cells are a big source of misery in many refinements. I have found the idea of WhatCheck to actually check your cell by looking at the projection of bond lengths of certain types along the cell axes

Re: [ccp4bb] unbiased electron density map

2008-01-10 Thread Dirk Kostrewa
Am 10.01.2008 um 01:53 schrieb Dale Tronrud: ... Your quote bring up another matter. An initial map, i.e. before refinement, is unbiased if it is an omit map. If you have done no refinement and you leave the interesting part out of the calculation of Fc there can not be any bias in either

Re: [ccp4bb] ideal bond length and bong angel

2008-01-10 Thread Gerard DVD Kleywegt
Please do NOT use the BB for abuse. thank you for your support. although i think some abuse of the english language is unavoidable in an international forum like this --gerard (more important issue: http://www.irrepressible.info/)

Re: [ccp4bb] crystallization robot

2008-01-10 Thread Boutin, Ray
Not to inject a commercial tone, but there seems to be a bias in the posting below. The ceramic tips are breakable, but not all find them incredibly so. And the price is less then $300, not $700. -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Lisa A

Re: [ccp4bb] crystallization robot

2008-01-10 Thread Alexey Rak
A little comment on not breakable Phoenix needles. I saw not recoverably bended tips (all 96!) as a result of not very careful operation (done by rep) during presentation of the instrument, I do not know how much is 96 nitinol tips set for replacement Additionally, you hardly can go with

Re: [ccp4bb] crystallization robot

2008-01-10 Thread Lisa A Nagy
I was wrong on the price- yes $300/per, but still, it was a lot of money when we had catastrophic crash several years ago (due to something going haywire- not a script or operator error). I know the nitonol tips are not fool-proof- they just seem a bit more forgiving than the ceramic tips. As

Re: [ccp4bb] Gordon conference on membrane protein structure/function

2008-01-10 Thread Susan Buchanan
Dear colleagues, We are pleased to announce that the Ligand Recognition and Molecular Gating GRC will be held in Ventura, CA, 2-7 March 2008. This Gordon Research Conference focuses on the structure and function of integral membrane proteins involved in transmembrane signaling (receptors)

Re: [ccp4bb] Zn fingers and Ni columns

2008-01-10 Thread Kendall Nettles
Phoebe, We were able to purify the estrogen receptor DNA binding domain with a 6his-tag. After cutting off the tag and re-applying to the Ni-NTA matrix, the protein did not stick to the beads. We had some low resolution crystals that contained a second protein and DNA, which suggests to me that

Re: [ccp4bb] unbiased electron density map

2008-01-10 Thread Dale Tronrud
Dirk Kostrewa wrote: Am 10.01.2008 um 01:53 schrieb Dale Tronrud: ... Your quote bring up another matter. An initial map, i.e. before refinement, is unbiased if it is an omit map. If you have done no refinement and you leave the interesting part out of the calculation of Fc there can not

[ccp4bb] Postdoc positions at Purdue University

2008-01-10 Thread Michael Oldham
Postdoctoral positions in Structural Biology are available immediately in the Chen laboratory at Purdue University. As part of the Markey Center for Structural Biology, the Chen lab integrates X-ray crystallography and biochemical methods to study the mechanism of active transport (See Nature,

[ccp4bb] ACA 2008 New Structures Session

2008-01-10 Thread Mark Wilson
This is an additional reminder to the community that January 12 (Saturday) is the deadline for abstract submissions for the New Structures session at the 2008 ACA meeting in Knoxville, TN. The session will feature a balance of talks from both the protein and nucleic acid crystallographic

Re: [ccp4bb] crystallization robot

2008-01-10 Thread Janet Newman
I bought a replacement Cartesian Tip (in Australia) on the 8th of october, 2007 and got charged $660 AUD (of which 60 bucks was the GST tax) - on the 8th of october, the AUD-USD exchange rate was 0.8956, so that would be $537 USD, which is quite a long way from both '$700' and 'under $300'. The

[ccp4bb] HHMI Post-doc Job opening

2008-01-10 Thread mjvdwoerd
All, I am posting on behalf of Dr. Karolin Luger. Dr. Luger has a job opening for a post-doctoral researcher. Job Summary: Looking for a highly motivated individual with a strong interest in integrated approaches to problems in structural biology. The lab has extensive crystallographic and

[ccp4bb] FW: [ccp4bb] crystallization robot

2008-01-10 Thread Bob Cudney
What a great time in crystallization; lots of options, lots of choices. After reviewing the automation available for crystallization and our unique needs we decided on the Phoenix. We have used the Phoenix from Art Robbins Instruments with excellent results since installation October 2006.

[ccp4bb] scale data with multiple MTZ files

2008-01-10 Thread Huiying Li
I tried to scale, using SCALA through CCP4i GUI, three blocks of data collected with one crystal (3 mtz files output from MOSFLM). The GUI has only one MTZ input slot. Which program can be used to combine the 3 unmerged mtz files together? CAD refused to handle these raw mtz files. Thanks in

[ccp4bb] crystallization robot

2008-01-10 Thread Kendall Nettles
We have been quite happy with our crystallization robot from Innovadyne. It transfers from deep well blocks to crystallization plates, and very reliably sets up 200nl + 200nl drops. We have tried smaller drops with decent success, which works better with certain plates. It can be easily

Re: [ccp4bb] scale data with multiple MTZ files

2008-01-10 Thread Roger Rowlett
Huiying Li wrote: I tried to scale, using SCALA through CCP4i GUI, three blocks of data collected with one crystal (3 mtz files output from MOSFLM). The GUI has only one MTZ input slot. Which program can be used to combine the 3 unmerged mtz files together? CAD refused to handle these raw mtz