Hi
First off, you really shouldn't be installing mosflm version 7.0.1 -
version 7.0.3 is the current one, and contains numerous bug fixes.
The developers are likely to meet any problem queries relating to
7.0.1 with "update to the current version first, then try again; if
the problem pers
> You would also need to change the top line of the file so
> that it was something like "#!/bin/bash -f", though I would
> be more inclined to use a proper traditional Bourne shell for
> this and use "#!/bin/sh -f" and use "set"s and "export"s throughout.
That's not actually necessary for a 's
Dear,
I'm intending to make an OMIT map using Sfcheck (ccp4i 6.0.2, Mac OS
X Tiger, PPC).
However, the program terminates with:
"has failed with error message
1525-005
1525-005
1525-005
1525-005
1525-005
1525-005
"
When using 'command line' from a script (with '-nomit 2 -map -origin -
out y
Hello,everyone!
I have a question,is the expression vector effect crystallization much?
For example,"PQE2-protein" structure has 2 molecules in one
unit-cell,"PQE70-protein single mutant" has 5molecules in one unit-cell.Is the
vector effect the results or the mutant itself?
Can pr
Dear List,
I am having a problem with running the latest version of pointless
(1.2.15) via ccp4i. This is with the new GUI task.
The logfile has the following, rather unhelpful information:
***
* Information from CC
杨柳青 wrote:
> Hello,everyone!
> I have a question,is the expression vector effect crystallization much?
> For example,"PQE2-protein" structure has 2 molecules in one
> unit-cell,"PQE70-protein single mutant" has 5molecules in one
> unit-cell.Is the vector effect the results or the mutant itself?
> C
I've had some problems getting recent versions of Pointless to link in a
portable way, due to a new call into the CCP4 library, & this can cause
segfaults on some systems. I keep hoping someone will tel me how to get
round this (a ccp4 routine calls getpwid which apparently cannot be
static)
You c
Installation of pointless-1.2.16 appears to have solved the problem.
On Thu, 2008-03-27 at 14:55 +, Phil Evans wrote:
> I've had some problems getting recent versions of Pointless to link in a
> portable way, due to a new call into the CCP4 library, & this can cause
> segfaults on some systems
I agree with Roger - provided, of course, that the same protein is
expressed from either vector with no extra amino acids at either end.
Quite often vectors include extra sequence at the N or C terminus that
are remnants of regions or linkers between the protein and
purification tags. The
Hi,Yang,
Tag(type and length) ,surface charge and crystallization condition are
parameters which affect the packing in the crystal. It should be the tag
or mutation not the the vector itself made you get different unit cell.
ta
liu
杨柳青 wrote:
> Hello,everyone!
> I have a question,is the expression
Hi All,
I'm having trouble putting an EM map into a large P1 box for molecular
replacement. We're trying to put density for a molecule about 300A in
size into a 1000A P1 box. Using maprot in cutting mode with a mask
yields a map with 1000A axes, but the extent of the map only covers
par
Hello,
Thank you very much for your help, now I setup Mosflm703 successfully. By the
way, can I remove the ccp4 program suite including previous version and
integrate the latest version into ccp4.
Thanks
Sincerely
Lu Yongzhi
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