[ccp4bb] prime-and-switch question

Hi Thomas，Hi all, I have used the amazing software prime-and-switch before,it is quite helpful. I have read through your article Using prime-and-switch phasing to reduce model bias in molecular replacement , but it is hard for me to understand. So what I am concerned is the basic

Re: [ccp4bb] prime-and-switch question

Dear Yolanda, Here is the basic idea of prime-and-switch phasing: We calculate phases from a model. These phases are mostly correct, but may have incorrect peaks at the coordinates of some misplaced atoms in the model. Next we are going to go through our reflections one by one. We calculate a

[ccp4bb] Registration deadline Workshop on Raw Image Format

Reminder: The registration deadline for the Workshop on Raw Image Formats in Structural Biology at BNL on 22 May 2008 is approaching. Please send email to [EMAIL PROTECTED] no later than 15 April 2008 if you are interested in attending.

[ccp4bb] Ligand Binding

Hi All, I want to confirm the binding of the substrate in the active site of the enzyme by soaking crystals of the apo-protein with the substrate. I generated a Fobs_lig - Fobs_apo difference electron density map. Inspection of the map did not show the characteristic electron density of the

Re: [ccp4bb] Ligand Binding

Rajan Pillai wrote: Can this problem arise due to loss of isomorphism in the crystals after soaking with the substrate? The c-axis dimension increased by ~10 angstroms Yes. -James Holton MAD Scientist