Dear All,
the consensus is that Cl- is most likely,
given the fact that backbone Ns and Arg
are involved in the contacts.
I think there is something wrong with my
surface calulation - need to check.
Thx! BR
_
From: Petri Kursula [mailto:[EMAIL PROTECTED]
Sent: Tuesday, May 27, 2008
Check the anomalous difference map on these sites.
Thanks
Abhinav
Abhinav Kumar
JCSG @ SSRL, MS 99
2575 Sand Hill Road
Menlo Park, CA 945025-7015
Phone: 650 926 2992
Fax: 650 926 3292
On May 27, 2008, at 6:38 PM, Bernhard Rupp wrote:
Dear All,
something one/some of you might have seen alr
Hey Joe,
set up a coarser grid around the native conditions. I know of one case
where semet crystallized nearly 2 full pH units lower than native. A
colleague made a couple of years ago a reasonable suggestion: don't do
s-met at all, do the crystal screening directly with se-met. Nowadays
certainl
Dear All,
something one/some of you might have seen already and
might know what it might be/how to analyse:
Material: dimer of Se-Met, Ni-purified, NaCl and NaAc in
purification history.
I have 4 'waters', 2 each in the same monomer location
in a dimer of 2.5A Se-Met structure. They are multipl
Novartis Oncology in Emeryville is seeking a highly motivated scientist
to contribute with Protein Biochemistry expertise to the pre-clinical
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The candidate should have extensive expertise in the area of Protein
purification and characterization. The i
Hi,
Unless you've already tried exhaustively - why not to try MIR, as you
mentioned - there are many derivatives out there to be tried and with a
small protein the chances are pretty good. All you need is one good
derivative :)
This certainly sounds easier than mutating exposed Met!
Cross-seedin
Have you thought about phasing off the sulfurs? This is quite a common
technique nowadays.
Jim
On Tue, 27 May 2008, Joe Smith wrote:
Dear all,
Sorry for an off-topic query.
I have been unable to crystallize a Se-met containing protein (8 Met
in 206 amino acids) in the native crystallization
Dear all,
Sorry for an off-topic query.
I have been unable to crystallize a Se-met containing protein (8 Met
in 206 amino acids) in the native crystallization condition ( 0.1 M
Tris pH 8.5, 1.2 M K-Na-tartrate; Theoretical pI of protein is 8.4).
As expected, solubility of Se-Met containing protein
Hi Juergen,
PROFESSS can be misused (?) to do this:
- just create a PDB file first the pattern atoms (numbered 1 to N)
and then the atoms of the substructure (numbered N+1 to M).
- you can then look for 'NCS' where only (1-N) atoms are related to
only (N+1-M) atoms.
Just run something l
Dear All,
This year's ACA is nearly upon us and CCP4 will again be running a
stand at the exhibition. Please come along to the stand where Francois
and myself will be there to answer any questions you may have about the
suite and demonstrate some of the new features in the upcoming CCP4 6.1
rele
In my experience it is still possible to do so, but that rather
depends from where you are flying. From my own experience it is always
good to contact the airport in question well in advance, partly to
check with them that it is ok, partly to let them know when you intend
to fly. This tends
Dear Tiancen,
I would look to the coordination of the Mn. In other words to the
geometry and distances from the Mn to it's potential ligands. The most
stable oxidation state of Mn is +2, with a octahedral (6-fold)
coordination sphere and bond lengths (e.g. Mn-O) of 2.1-2.4 Å. For Mn3+
one wou
Dear all,
given a very overcrowded substucture (e.g. from SHELX, PnB for sulfur)
in a big asymmetric unit (over 100 proposed S),
given the resolution used for the substructur determination,
given several possible S-patterns (taken from PDB), like PAN-pattern,
Apple-pattern a.s.o. as a pdb-file.
PROTEROS is a leading service-provider for the preparation and
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Dear all,
I wonder if it would be still/again possible to check in a dry shipper for a
flight inside Europe. What is your experience?
Cheers,
Clemens
Dear all,
Sorry for the non-topic question. We are working on an enzyme with two Mn2+
in its active center. The Mn2+-coordinated water molecules are responsible
for binding and nucleophillic attack of the substrate. We soaked the protein
crystal with an inhibitor and determined the structure, in w
Vellieux Frederic schrieb:
Dear All,
Would anyone know where to find the latest stable release of Coot for
Fedora Core 3? I tried to access the pages ~emsley on the York ysbl
server, but to no avail (You don't have permission to access
/~emsley/coot/ on this server.).
Thank you in advance,
Dear All,
Would anyone know where to find the latest stable release of Coot for
Fedora Core 3? I tried to access the pages ~emsley on the York ysbl
server, but to no avail (You don't have permission to access
/~emsley/coot/ on this server.).
Thank you in advance,
Fred.
begin:vcard
fn:Fred.
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