Dear ccp4 users:
Where can I find a description of the program Mapmask (Patterson): There
are several possibilities to calculate a map (Patterson, Patterson
from intensity, difference patterson, anomalous difference
patterson, Patterson using anom. difference data).. What are the
differences
I've used 2 M KCl and 3 M NaCl without any trouble. Notably, you can use
urea as well for denatured proteins. I've purified histones on HisSELECT w/o
any trouble and they have a pI of 11. Naturally, His-tag is a bit of an
overkill for histones and such since they're purifiable over CM columns at
Dear CCP4ers
can anyone recommend papers describing crystal structures of proteins
with a large functionally important disordered domain or domains.
Thanks in advance
Gina