[ccp4bb] Disordered domains in crystal strutures

Dear CCP4ers can anyone recommend papers describing crystal structures of proteins with a large functionally important disordered domain or domains. Thanks in advance Gina

Re: [ccp4bb] Binding to Nickel in the presence of SDS or arginine

I've used 2 M KCl and 3 M NaCl without any trouble. Notably, you can use urea as well for denatured proteins. I've purified histones on HisSELECT w/o any trouble and they have a pI of 11. Naturally, His-tag is a bit of an overkill for histones and such since they're purifiable over CM columns at pH

Re: [ccp4bb] Binding to Nickel in the presence of SDS or arginine

Hi, Does high salt concentration(around 0.7M Nacl) in NiNTA elution do better. And what about the purification of high positively charged protein (like PI 10) in NiNTA. Thanks Debajyoti Dutta On Sat, 28 Jun 2008 Artem Evdokimov wrote : >Ni salt of dodecyl sulphate is not soluble. Therefor

[ccp4bb] Mapmask (Patterson)

Dear ccp4 users: Where can I find a description of the program Mapmask (Patterson): There are several possibilities to calculate a map ("Patterson", "Patterson from intensity", "difference patterson", "anomalous difference patterson", "Patterson using anom. difference data").. What are the dif