Re: [ccp4bb] sequential renumbering of a messed up pdb file

2008-08-04 Thread Eleanor Dodson
It is an absolute pain! do you mean you have residue 1 A 1A 2A 2A 3A 3A etc where they should be numbered 1 2 3 4 5 6 A? or should it be 1A 1B 2A 2B 3A 3B etc? Eleanor William Scott wrote: Hi folks: I am hoping there is a simple answer I have overlooked to the following question. I have

[ccp4bb] R-axis HTC set of images

2008-08-04 Thread Andrew Leslie
Dear All, In order to help calibrate some internal parameters in MOSFLM, I would greatly appreciate a dataset of images collected on an R-axis HTC from a well diffracting sample (such as lysozyme, insulin etc) on a laboratory source. Ideally I would like a complete dataset, preferably collect

[ccp4bb] Postdoc and PhD Student Positions in Electron Microscopy

2008-08-04 Thread Haixin Sui
Applications are invited for two open positions in the laboratory of Haixin Sui in the Wadsworth Center, Albany, NY. One opening is for a postdoctoral fellow starting in September of 2008. The other is for a Ph.D. student starting in the spring or fall of 2009. These are funded positions adm

[ccp4bb] R-axis HTC set of images .. got some

2008-08-04 Thread Andrew Leslie
Many thanks to those who offered datasets, I think that I am well set up now. Thanks again, Andrew

[ccp4bb] protein crystal decay problem

2008-08-04 Thread lei feng
hello everyone, I am seeking help on a crystal decay problem I got crystals of a protein , but it decayed very fast in syncrotron beam. From the initial index, it is P222 space group, I need about 180 images, its initial diffraction went to about 3 A, and after 70-80 images, it droped to abo

Re: [ccp4bb] protein crystal decay problem

2008-08-04 Thread junfeng liu
How about 90? If it is ok , maybe you need more than 3 or more xtals to collect the whole dataset and merge them together. Otherwise your xtals should be the anisotropic .You'd better to collect images in the better region or screen more xtals to get better ones. Good luck! leo lei feng wrote:

Re: [ccp4bb] protein crystal decay problem

2008-08-04 Thread Juergen Bosch
I'd rather go for a complete low resolution dataset. Is this a native crystal ? HA derivative ? Even with a 4Å data set you can do something it's painful but not impossible e.g. molecular replacement if possible. Then at least you would know your spacegroup & perhaps gain some insights from