Hi Peter,
At APS/SBC sector 19 all shipping dewars are turned upside down until all
LN2 drains out, this is repeated 2-3x times to remove the residual caught in
the top. With this method there is no free LN2 remaining in the dewars to
leak out. During the upside down tipping the dewars must not be
A postdoctoral position is available to continue our study on the crystal
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(Structure. 2008;16:597-606). This is the protein missing in hemophilia. The
candidate should have a sound knowledge and experience in protein X-ray
crystallo
Hi folks
that should have read "OS X .app (i.e.)"
On 26 Aug 2008, at 03:03, harry powell wrote:
If there is demand for an OS X (i.e. something that you double
click the icon to run) I can supply
Harry
--
Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre,
Hills Road, C
Hi
It looks as if you are trying to run Mosflm as a Mac application (or
".app", for the cognoscenti); the short answer is that it isn't one,
and that you run it as an X11 command-line program. You can't double
click the icon to run it even if you associate it with an application
like xter
Likewise, incorporation of 30% (or even more) of ethylene glycol,
polyethylene glycol, propylene glycol etc. may save you from proteolysis.
This did not work for me but a friend of mine has had very good luck with
it. Kind of hit/miss method, though.
Artem
-Original Message-
From: CCP4 bu
Dear all,
Due to the presence of residual liquid nitrogen in dry shippers
'steaming' out of a tipped-over dewar on a Fedex dock, we (PX-ers in
the ALS) have been placed under some scrutiny with regards to
dry-shipping dewars. In particular, I am interested in how people
empty their dewars/pucks/vi
Hi there! Real noobie question here- I am hoping someone can help me.
I have been trying to download and install MOSFLM from the pre-built
versions here:
http://www.mrc-lmb.cam.ac.uk/harry/mosflm/ver703/pre-built/index.html
Specifically, I am trying to install the "Universal" version for OSX
(al
Glad it worked.
I should have written
"sudo /bin/bash"
or
"sudo /bin/zsh"
or
"sudo /bin/tcsh"
followed by sourcing the appropriate startup script.
However, if I run the tcsh command, I get this error:
limit: stacksize: Can't remove limit (Invalid argument)
oddly, this does not happen wit
Hello Everyone!...
I'm starting to thing about an air conditioning system for the optical
hutch of one of our beamlines, which suffers from positional and energy
drifts associated with several sources.
It's clear that the specifications for such system can cover a wide
range of requirements. H
A postdoctoral position is available immediately to study the structure and
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area, involving actin-binding proteins, signaling proteins, cytoskeleton
scaffolding and membrane binding proteins. Most of the projects deal with
p
George & Phil -
Having slept on this it's beginning to dawn on me that George is right
and my assertion that is the appropriate data to use in MR and
F^2-based refinement was incorrect. However my assertion that is
the best estimate of F^2 (and that is the best estimate of F) is
still valid.
Thanks Herman, George and Leo.I will try the methods.Their suggestions are
summarized below.
Dear Hongnan--
On 25 Aug 2008, at 17:47, conancao wrote:Or does any program could refine the
the same ligand with 2 different orientations into a mixed electron density?
That's an easy
onehttp://scr
A position is available for a post-doctoral researcher (or Ph.D.
student) at the Laboratory for Structural Neurobiology at the
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Thanks a lot for this hint: it works! Including REFMAC, which ran to
completion and used Arp/Warp to add waters successfully.
Anita
Le 25 août 08 à 16:04, William G. Scott a écrit :
Under 10.5.X, sudo doesn't inherit the environment. So the best
thing to do is start a root process like this:
Hi Alex,
Such gels were used to separate in the past 70S ribosome, plus/minus one
protein - so separation is excellent. Cannot recall the references but
papers published in the 70s' I believe.
The agaose is used to provide "strength" to low percentage acrylamide, say
3% acrylamide/0.5% agarose. J
Hello,
I have a question about comparing anisotropic displacement parameters for a
series of 6 mutant and a wt structure with diffraction from 1.2-1.5 Å. The
space group and unit cell for the series is the same, and most xtals were
obtained by seeding from a hit for one mutant. There are two
Hi Debajyoti,
There is another simple thing you can try: Raise your NaCl concentration
to 500 or 1000 mM in your lysis buffer. This helps to clean up your
sample further and it might inhibit proteases in your lysate.
Good luck,
christian
Debajyoti Dutta wrote:
Hi,
This is going to be an
Under 10.5.X, sudo doesn't inherit the environment. So the best thing
to do is start a root process like this:
/bin/bash
(or /bin/zsh or /bin/tcsh )
source /usr/local/ccp4-6.0.2/include/ccp4.setup-bash
(or whatever it is for your shell)
Then issue ccp4i
William G. Scott
Contact info:
htt
Dear Friends and scientists,
I know An inhibitor(Say A) that acts as an anti destabilizing agent for a
homotetrameric protein.
that is the disturbance in the tetramer leads to aggregation of the protein
,this inhibitor A stabilizes this disturbancethis is one story.
the other, recently we wer
Dear All,
Thanks for your suggestions about software to map out the secondary
structure of a protein onto a line.
I've compiled all of the suggestions into one list:
http://tardis.nibio.go.jp/joy/
http://www.biochem.ucl.ac.uk/~roman/procheck/manual/examples/plot_06.html
http://webclu.bio.wzw
My faithful old Mac G5 siezed up and I have to use a brand new MacBook
Pro from now on. It's an Intel machine with Leopard.
I managed to install CCP4 together with TLK/BLK programmes. After a
long while searching for bltwish routines I discovered that they are
automatically installed in /us
Dear Ian and Phil,
I am very reluctant to touch the experimental data, so I am a
bit concerned about the argument that it is better to refine
against than Imeas. Are you sure that the 'best estimate'
of the intensity is also unbiassed?
To take an extreme example, suppose that we have processed
SHELXL has been able to refine such occupancies (e.g. as p and 1-p for the
two ligands, i.e. one extra parameter) for many years, and if I have
understood correctly last week's version of phenix_refine can do so too.
George
Prof. George M. Sheldrick FRS
Dept. Structural Chemistry,
University o
Dear all:
In a 1.8A protein crystal structure,I find clear extra positive
electron density suggesting 2 possible different orientations of the same
ligand at a single active site.I identify those two orientations by mannually
fitting 2 orientations and then refining them separately.
You're right ^2 !=
It wouldn't be hard to get Truncate to output as well
One reason why I've got rid of the Is in mtz files myself is just to
save space (less important these days), particularly if I knew I
wasn't going to use them. If we add then we have for each dataset
F, Dano, F+,
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