Thanks to all who responded to my question regarding the energetics of a
known interface applied to orthogolous dimers.
Steven Darnell asked me for some clarifications. I have the structure
of a homodimer, defined the dimerization interface and substituted the
residues at said interface with
Dear All
I want to create a random crystallization screen using
given set of crystallization parameters (pH,
Precipitant, salt, additive.) for protein-DNA
complex crystallization. I was using Brent Segelke's
CRYSTOOL program, not accessible online now. Please
can you suggest any other such
Alan Fersht: Enzyme structure and mechanism.
Definitely!
Nadir Mrabet
--
Pr. Nadir T. Mrabet
Cellular Molecular Biochemistry
INSERM U-724
Nancy University, School of Medicine
9, Avenue de la Foret de Haye, BP 184
54505 Vandoeuvre-les-Nancy Cedex
France
Phone: +33
Dear Rajakumara
I forgot to say, there's a very simple way to do this by aliasing.
You write (let's say by hand) one or more random screens with solutions
called e.g. Precipitate1, Precipitate2, PrecipitateN; Buffer1 to
BufferN; Additive1 to AdditiveN etc.
Each time you want to make a
On Sun, Aug 24, 2008 at 08:08:00PM +0100, Phil Evans wrote:
As you say, the linear fit is only used to put data on a very rough
absolute scale. This isn't necessary, but it doesn't hurt
It might be necessary after all for experimental phasing using heavy
atoms: you want to start refinement of
Andreas,
Thanks for clarifying the situation. I have one other suggestion you can
think about based on your description of a monomeric hybrid. Since you
are mutating your original yeast protein sequence to human/yeast
chimeras, you could directly model the mutations in Rosetta and skip the
I would have thought that it would always be a good idea to refine only
the occupancies in the first few cycles and only refine co-ords B
factors once the occupancies have settled down to sensible values. But
in that case wouldn't the Fcalc's be linearly dependent on the
occupancies so the
Dear Ian,
On Fri, Sep 12, 2008 at 05:53:35PM +0100, Ian Tickle wrote:
I would have thought that it would always be a good idea to refine only
the occupancies in the first few cycles and only refine co-ords B
factors once the occupancies have settled down to sensible values.
Correct, this
For low resolution data (~7-8 Angstroms or so), there are cases where
any type of occupancy refinement isn't tremendously stable. I'm much
less bothered by occupancies1 (for phasing sites) than I am with cases
where all of the occupancies are refined to 0 (especially when fixing
the occupancies
Dear Hao --
On 10 Sep 2008, at 02:00, jxqi wrote:
Does anyone have the experience of using Phoenix robot
automatically setup the
crystal? Which plate the Phoenix could use such as 96-well sitting
drop or
hanging drop? If available could you also tell me the CAT NO. of
hampton!!!
Just to
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