Hi Sampath, We had a very similar case, an octamer (422 symmetry) in C2 space group. What saved us for solving the structure (and by the way interpreting the self-rotation function !!) was a low resolution electron microscopy envelope (silver stain and cryo-EM) that gave us the organisation of the octamer. Sure, the condition is that the content of the asymetric unit corresponds to what you have in solution. I would bet you have 6 or 9 molecules in the asymmetric unit corresponding to very low or very solvent content.
Good luck, Claudine > Dear All, > > I have a problem in analyze the self rotation function. My crystal is > belongs to C2 space group. According to Mathews calculation, this crystal > shows 7 or 8 molecules in the asymmetric unit. But I couldn't conclude the > number molecules in the A.U. So I calculated the self rotation function > using the program MOLREF. But Still I am not able to interpret the results > using that. Here I'm attaching the figures which I calculated the self > rotation function with different chi angles. Could anyone help me to find > the symmetry of molecules in the A.U? It will be great helping me to solve > the structure. > > Thanking you, > > Sincerely > > Sampath > ***************************************************** ATTENTION : NOUVELLE ADRESSE MAIL : [EMAIL PROTECTED] ***************************************************** Pr Claudine MAYER Université Paris Diderot - Paris 7 *****************************************************
