Thanks to all for your help and suggestions. We are still working out
the best protocol for our specific protein, but I list here the
suggestions received, since they may prove helpful to others as well.
1. I assume that your native (12 Cys) protein runs as monomer on
non-reducing SDS-PAGE and
Hello all,
A pharmaceutical lab near us has closed and we are trying to find a new home
for their Rigaku protein crystallography system. It is complete with an RU-H3R
generator, R-Axis IV detector, Blue optics system, and X-stream. It was
under continuous service contract until July 2008 and i
Dear All,
Is there any alternative program to Nucplot for generating nice
schematic diagrams
of protein-nucleic acid interactions?
Thanks!
Martin
It is hard to say without the program output as well, and information
about the unit cell.
However it seems that you have a three fold axis at 120 degrees to the
C2 2fold axis,
and 3 other NCS 2-folds perpendicular that 3 fold.
It is possible your space group is really H32 - run your data (eith
