you need to select the Free R in the highest possible Laue group , then
use cad to extend this to all reflections in the chosen point group.
This is best done immediately post-truncate..
eg if you twinning operator is k,h,-l, then if
1,2, 3 is assigned to the free set so should 2, 1, -3 be.
A good compromise in the case of suspected perfect merohedral twinning
is to select the free R reflections using thin shells. Since the twin
related reflections have the same sin(theta)/lambda values they will
not be split over the working and reference sets. XPREP offers this
option. A
nanodrop system is wonderful. it helps very much in solutions with
high interference from detergents etc etc.
i highly receommend nanodrop specs
PSP
On Thu, Dec 4, 2008 at 10:16 AM, Tim Gruene [EMAIL PROTECTED] wrote:
Dear all,
we would like to purchase a UV spectrometer for measuring
I also have to come in defense of the nanodrop here.
I have measured up to A280 = 98 and the curve is always reasonably
smooth, spikes normally mean that bubbles have formed. And proper
cleaning seems to be rubbing with a kimwipe three or four times
after each drop. If the last user does
Getting even further off topic, we had a Nanodrop demo for trial shortly
after hearing that a significant number of crystallization groups were
using them. I won't go into the trial details, but I'd be interested in
hearing from people (via email rather then the ccp4 postings) who did
systematic
I am trying to build a model of a 6 Da protein from the diffraction data
collected at 2.0 A resolution. There is a 10-residue stretch that has such bad
electron density that even at 0.4 sigma level one can hardly see any well
defined density for residues with long side chains.
My question
This might help:
Acta Cryst. (1997). D53, 540-543
Local Improvement of Electron-Density Maps
Pavel.
PS It will be implemented in PHENIX sometime in future, but for the
moment you will need to so some scripting.
On 12/8/2008 11:08 AM, Andy Millston wrote:
I am trying to build a model of
Hi all,
We have available a complete Rigaku crystallography system, purchased in
2002. It includes a high-brilliance FR-D generator, an R-Axis IV++ detector,
Mirror Confocal Purple optics, X-Stream 2000, Haskris chiller, and all
accompanying accessories and software. Please contact us for
Hauptman-Woodward Medical Research Institute
Virus-Host Interactions and Structures
The laboratories of Drs. L. Wayne Schultz and Tim Umland are seeking highly
motivated individuals to participate in a joint interdisciplinary research
project investigating virus-host protein-protein
There are certainly reasons why it shouldn't work:
- the protein may alter the structure - even if its not a
big bender, it may tweak the groove widths or introduce a
shallow overall bend in a long binding site
- the phosphates move the most between DNA structures, and
unfortunately they also
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