4) You do not need buy hard drives, DVDs, .. and no need in suffering from
different format of files, ...
-disk space is cheap and becoming cheaper
And what about different format of pucks/cassettes/tongs etc?
I can still read my old Vax backup tapes (on a linux box with an
exabyte tape driv
I would be against the storage of crystals due to a number of reasons.
0) Crystal is harder to be fake than images; From crystal to images,
maybe only 1 hour needed;
-would be worried about: proper handling of crystals after data collection,
ice formation, dropping, remounting if it another futur
On Monday 23 March 2009 14:34:24 Peter Zwart wrote:
> Hi,
> >
> > If we then require that the rms change in intensity be greater than the
> > average noise, then we can write down the requirement:
> > sigma(I_P) < rms(deltaI)
> >
> .
> > I_P/sigma(I_P) > 1.3*sqrt(MW/N_H)/fpp
>
> one can actually r
Hi,
>
> If we then require that the rms change in intensity be greater than the
> average noise, then we can write down the requirement:
> sigma(I_P) < rms(deltaI)
>
.
.
.
> I_P/sigma(I_P) > 1.3*sqrt(MW/N_H)/fpp
one can actually require that
abs(delta I) > 3 sigma(delta I)
Using eq. 13 from Ac
All,
Just wanted to point out that Formulatrix is interested in donating an
imager plus software to a lab conducting research to advance the human
condition, including cancer and other disease research.
More info on http://www.formulatrix.com/donation.shtml
Flip
Here is correct website
http://www.ccp4.ac.uk/schools/APS-2009/
Sorry for confusion,
N.
Ruslan Sanishvili (Nukri), Ph.D.
GM/CA-CAT
Biosciences Division, ANL
9700 S. Cass Ave.
Argonne, IL 60439
Tel: (630)252-0665
Fax: (630)252-0667
rsanishv...@anl.gov
-Original Message-
From: CCP4 bull
This is 2nd announcement of the CCP4 workshop entitled "CCP4 school:
"From data collection to structure refinement and beyond" and to be held
in Argonne National Laboratory, near Chicago.
A full program and list of speakers is now available. These and other
details (application process, accommodat
Hi,
Which secretion signal(s) did you use? The choice of secretion signal can
make or break the entire process.
Have you checked total yield of your protein in the cytoplasm and in the
medium? Can you make assumptions regarding the bottleneck - is it protein
synthesis/folding or is it the secreti
Hello Chen,
You did not mention perhaps the most important element that can make or
break the secretion process - namely the secretion signal. Which signal(s)
have you already tried?
Have you attempted to quantify the protein made in the total IC? In other
terms, is the bottleneck in the protein
Hi,
We are trying to express a protein in insect cells as secreted. However, the
yield is VERY low using either pFastBAC or pAcGP67A. We have tried many
different constructs and this is the only one that at least expresses. I wonder
whether anyone knows how to improve. Many thanks.
Best,
Ch
On Mon, 23 Mar 2009, Phil Evans wrote:
I'm happy to change the column titles if it makes it clearer. Actually the
"I/sigma" column in the Scala output is not very useful:
it is / RMSscatter, ie the mean intensity/mean error, for individual
observations, not taking into account multiple measur
I'm happy to change the column titles if it makes it clearer. Actually
the "I/sigma" column in the Scala output is not very useful:
it is / RMSscatter, ie the mean intensity/mean error, for
individual observations, not taking into account multiple
measurements. Because it is ratio of means
I guess when I talk about signal-to-noise I assume the one that is most
relevant to the task at hand. So, to me, I/sigma(I) at the phasing step
would be the average intensity (I) divided by the sigma (standard
deviation) assigned to it AFTER scaling/mergeing. I admit that the
"I/sigma" column
I like to think of things in terms of signal-to-noise, and one can
use a
rearrangement of the Crick-Magdoff equation to tell you what the I/
sigma
of your data set needs to be for delta-F to be greater than
sigma(delta-F):
I/sigma(I) > 1.3*sqrt(Daltons/sites)/f"
where:
I/sigma(I) is the sig
Hi Joe
The problem is that your dataset name contains colons, which Windows
does not like. This is probably because your original mtz file was
generated using scalepack2mtz or dtrek2mtz and you did not explicitly
set a dataset name, so that a default was chosen based on the date and
time and usin
Miguel,
CCP4 are intending a major project on the ccp4i database and one of
the requirements will certainly be enabling multi-user access to
databases. I'm sure we will be looking for your input on other
requirements when we have someone in post.
Liz
On 22 Mar 2009, at 08:11, Miguel Orti
Contact in CCP4i can calculate the distance between specified
chains/residues. Pymol can display hydrogen bonds too.
Joe
peter hudson wrote:
Hello all
I have a very quick question. Is there any programme, which can
calculate the H-bond pattern or residues involved in H-bonds formation
betw
sorry ... user error ... input is case sensitive ... :-)
ingo
>it seems you have a version of coot in there, where the "get monomer"
>via 3-letter code is broken. alternatively "search monomer" does work,
>you can click on the molecule of choice and it gets nicely imported ...
>
>not sure whether
it seems you have a version of coot in there, where the "get monomer"
via 3-letter code is broken. alternatively "search monomer" does work,
you can click on the molecule of choice and it gets nicely imported ...
not sure whether there is a newer version that would work ...
cheers
ingo
--
CREL
This is VERY VERY VERY irritating!
Why has it been allowed...
Is there any advantages??
Eleanor#
You don't necessarily need, or want, a program to do it.
The trick is to determine exactly which points in space you want to use.
If you can select a start and end point for each of your strands and
record their x,y and z, that gives you vectors you can apply some basic
vector geometry to, e.g
Hi all,
is there a similar software for beta strands ?
thanks
2009/3/23 Guenter Fritz
> Hi Peter,
>
> you can do that with interhlx:
> http://nmr.uhnres.utoronto.ca/ikura/resources/data+sw/interhlx/
>
> There is also a way to determine the interhelical angle with molmol,
> http://hugin.ethz.ch
Hi Peter,
you can do that with interhlx:
http://nmr.uhnres.utoronto.ca/ikura/resources/data+sw/interhlx/
There is also a way to determine the interhelical angle with molmol,
http://hugin.ethz.ch/wuthrich/software/molmol/
defining cylinders for the helices.
HTH
Guenter
Hi all
I would be interes
Hi all
I would be interested to know about the programmes which can calculate the
angle between the two helices. I want to calculate the angle between the
two different helices from two different subunits of the structure. I know a
non-supported programme at ccp4 called helixang. Is this programm
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