Have you tried DMMULTI? It can do a brilliant job..
Eleanor
Ethan Merritt wrote:
Hi all,
I have an interesting problem case at the moment.
We have crystallized the same 2-domain protein in two different,
i.e. non-isomorphous, crystal forms.
There is a decent homologous structure for one
Ethan Merritt schrieb:
Hi all,
I have an interesting problem case at the moment.
We have crystallized the same 2-domain protein in two different,
i.e. non-isomorphous, crystal forms.
There is a decent homologous structure for one domain
(about 40% of the total), but no known homologous
Ambrose Cole wrote:
I've found out how to do it on ccp4mg but I was working with the new QtMG,
still can't find where to do it there though.
Ambrose
Dear Ambrose,
There should be an option Colour browser.. on the colour menu. Click
on the Create colours tab to add whatever colour you like.
Dear all,
Are you tired of protein expression and purification? Want to concentrate on
Crystallisation and Crystallography only?
Are you interested in working in a very lively and young company with about 45
colleagues of talented scientists and technical staff in a pleasant atmosphere?
Only problem: it's in Bavaria... and we belive that you will need an
excellent command of the German language both written and spoken to work
here efficiently and live here happily.
Dear das Doktor von Hannes!
Ich das Jobb very interessiert ist worden worden geworden. Die Bayern
placement
Gerard, I think you have no time left to enjoy the beer and discuss in
the native language with the bavarians
With about five structures a day, you will have your hands full on doing
a good job if you work to fast and leave it up to automation, you
are bound to miss the bound
Dear all,
i am working on a structure of M.tb, solved at 2.18 A. In my structure there
is a density near to the active site and we modelled it as a malonate (C3 O4
H2) molecule acorrding to the density, which was not used in any of our
buffer except we used 10 mM DTT, 1.6 M Ammonium sulphate, 100
Theoretically, citric acid can decompose into beta-ketoglutaric acid, and
the latter may decarboxylate into malonic acid...
Not sure if this happens in aqueous solutions, but if your citrate had
malonate contamination and your enzyme prefers malonate to anything else -
that's where it may have
To all colleagues at CCP4BB:
We have the following X-ray diffraction equipment to give away:
I. Siemens X-1000 Multiwire Area Detector. It has been manufactured by
Siemens Analytical X-ray Instruments Inc. in Madison, Wisconsin, USA.
Equipment available includes multiwire area detector
