Hi Toyoyuki,
If your protein bind to metal ions you could try low concentration of
chelating agents in the purification and storage buffer. Take a look at the
following reference:
Chelating Agents Stabilize the Monomeric State of the Zinc Binding Human
Papillomavirus 16 E6 Oncoprotein
Degenkolbe
Dear Ose,
I think we have met similar problems. My protein is relatively small, ~17kD,
but it could form aggregates larger than 100nm. A classic Protein Science
paper in 2003 by DR Smith titled "Crystal structures of fusion proteins with
large-affinity tags" offered me great help. With MBP tag and
Dear James,
Could you provide the reference of your success story? My protein also
formed large soluble aggregates and I am desperate for such successful
stories!
By the way, have your performed DLS to your protein? What about the
polydispensity? Is it lower than 20%?
Thanks in advance!
Sincere
Does it work with NFS v.3?
Cheers.
--Original Message--
From: David Jones
Sender: CCP4 bulletin board
To: CCP4BB@JISCMAIL.AC.UK
ReplyTo: David Jones
Subject: [ccp4bb] Refmac Problem - Permission Denied
Sent: Aug 29, 2009 3:32 PM
I would appreciate if any one has any ideas on this
I hav
I would appreciate if any one has any ideas on this
I have a strange NFS related problem, if anyone has any ideas...
I am running refmac with the ccp4 directories mounted on an nfs
share (v4) mounted under /usr/local
I get the following error...
#CCP4I TERMINATION STATUS 0 Last system er
Dear Eric,
Thank you very much for your suggestion. It worked.
Dear Paul,
Thank you for your kind offer with the help (and for writing such a wonderful
program + providing users with support!). I will compare the cif files and see
if I can pick out the differences...
Sincerely,
Peter
Hi
