Kjeldgaard Morten wrote:
On 14/11/2009, at 20.17, Miguel Ortiz Lombardia wrote:
Le 14 nov. 09 à 19:15, Kjeldgaard Morten a écrit :
On 14/11/2009, at 18.55, Ronald E Stenkamp wrote:
The rumblings here at the Univ. of Washington among the
computational modelers is that some of their current m
Hi Jeremiah -- the short answer is Yes You Must! Don't whether you need
that high concentration generally, but if it's not too osmotically
active, the crystals should be fine; test it.
But when mounting SeMet crystals, it's always a good idea to add fresh
DTT or TCEP or whatever, it seems to
We are trying to crystallize a small fragment (approximately 120aa) of a
membrane protein. We encounter many problems in solubilizing the protein and
would like to know if any one has experience in crystallizing proteins in
the presence of TFE (Tetrafluoroethylene) (I understand that high
concentra
All true - but knowing that unit cell is stored as text, direct edit
seems to require fewer keystrokes. Obviously, this is so that I can
happily use the keystrokes I saved to write this :)
On Sun, 2009-11-15 at 23:01 +, martyn.w...@stfc.ac.uk wrote:
> Well, yes, that's how I would do it (but
Hi Jacob,
an alternative explenation might be your protein does not bind via the
tag to the resin but unspecifically. What happens if you add the
recommended NaCl concentration? Does it fall off too?
Just a thought,
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of
Hello Jacob,
I am not entirely sure why this has happened to you...
A couple of suggestions that may help:
1. dial down the TRIS to 20 mM - you can also use TRIS-HCl (unless the
second buffer is there for some other reason).
2. try a 'stronger' resin. I know in the past I've always strongly advo
The Stroud Lab at UCSF (http://www.msg.ucsf.edu/stroud/index.htm) is looking to
fill multiple post-doctoral positions in 1. membrane protein crystallography
and 2. structure and function of RNA-modifying enzymes.
Applicants should have background in biochemistry, molecular biology, and/or
cryst
I wanted to update refmac5 to the latest version in CCP4. I downloaded
v5.5.0105 and unpacked the tarball. The three files are named
refmacgfortran, libcheckgfortran and makecifgfortran. So, I moved the
corresponding files refmac5, libcheck and makecif in ccp4-6.1.2/bin/ to
another folder an
Dear Crystallographers,
Recently I incubated a small amount of His-select resin with bacterial
lysate in
(50 mM TRIS-HEPES pH 8.0, 10 mM CaCl2),
and washed the resin, for certain experimental reasons, with
(50 mM TRIS-HEPES pH 8.0, 0.2 mM CaCl2),
at which point a significant amount of my pr
Dear All,
A quick reminder to you all about this coming January's CCP4 Study Weekend
entitled "From Crystal to Structure with CCP4" (January 6th-8th 2010). The
deadline for the first round of registrations will be the 21st of November
after which the registration price will increase from £210 t
Hello everyone,
Perhaps this is a newbie question, so I apologize.
After much work and a lot of thanks to the ccp4 bulletin board I just tried to
submit my first structure to the pdb database. It is a membrane protein and
therefore has some detergent molecules bound to
The rumblings here at the Univ. of Washington among the computational modelers is that
some of their current models might be more representative of protein structures in
solution than are the crystal structure models. It may take less than a "couple of
decades" for a reduced emphasis on crysta
Refmac refines against the original twinned data.
Eleanor
Bernhard Rupp wrote:
Dear All,
following the twinning threads as well as the refmac manual it is
not quite clear to me yet whether refmac finds the twin operator and
fraction, detwins the data, and refines against those detwinned data,
There are many and various programs that do this.
As Martin says - cad will do it - you can use the GUI to change each
data set in the file..
if there is only one data set the easiest is this:
mtzutils hklin old.mtz hklout new.mtz
CELL 168.981 168.981 168.981 90 90 90
end
eleanor
martyn.
To all colleagues at CCP4BB:
We have the following X-ray diffraction equipment to give away:
I. Siemens X-1000 Multiwire Area Detector. It has been manufactured by
Siemens Analytical X-ray Instruments Inc. in Madison, Wisconsin, USA.
Equipment available includes multiwire area detector X-10
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