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Two Postdoctoral Associate positions are immediately available in the
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You can include urea in your gel to resolve ~5 kDa without using
gradient gel. I am sorry that I forgot what was the percentage. Please
take a look at "Gel Electrophoresis of Proteins: A Practical
Approach". Or you can try the premade gradient gel from Invitrogen.
http://www.invitrogen.com/etc/medi
I have had very good results using 10% Tris-tricine gels, following the
protocol in Current Protocols for our chemokines (~8,000 mw)
James W. Murphy, Ph.D.
Associate Research Scientist; Dept. of Pharamcology
Facility Manager; Macromolecular Crystallography Facility
Yale University School of Medi
Try high percentage Tricine gels...
Robert Keenan
Assistant Professor
Dept. of Biochemistry & Molecular Biology
GCIS W238
University of Chicago
929 East 57th Street
Chicago, IL 60637
(o) 773.834.2292
(f) 773.834.5416
(e) bkee...@uchicago.edu
http://keenanla
Dear Crystallographers,
does anybody have any good tricks for getting nice, sharp SDS-PAGE bands of
peptides < 10kD?
Regards,
Jacob Keller
***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1
Thank you - yes, this site also contains the two links to the useful
sites Ligand Expo and PDBeChem.
Dirk.
Am 11.02.10 13:40, schrieb Konrad Hinsen:
On 11.02.2010, at 10:37, Dirk Kostrewa wrote:
I've found this link already, but it is not very useful, because it only
generally describes
On 11.02.2010, at 10:37, Dirk Kostrewa wrote:
> I've found this link already, but it is not very useful, because it only
> generally describes the format. What is missing, is a good actual dictionary
> of the usual compounds (amino acids, nucleic acids, and so on). In the
> coordinate section,
If you want dictionary, atom naming and everything else about new
compound names you can copy all "monomers" (in our definition) from:
ftp://ftp.ebi.ac.uk/pub/databases/rcsb/pdb-remediated/data/monomers/components.cif.gz
It has all compounds used in pdb with "idealised" coordinates,
compound
Thurs. Feb. 11th 2010
EBI
Dear Dirk
I also put to the PIs your suggestion of web-site-service, will keep you
and the rest of the community update.
Miri
On Thu, 11 Feb 2010, Dirk Kostrewa wrote:
Dear CCP4ers,
Kim Henrick from EBI sent me pointers to
http://ligand-expo.rcsb.org/ld-search.h
Dear CCP4ers,
Kim Henrick from EBI sent me pointers to
http://ligand-expo.rcsb.org/ld-search.html
and to
http://www.ebi.ac.uk/msd-srv/msdchem/cgi-bin/cgi.pl
for looking up reference dictionaries. Especially, the first link turns
out to be a gorgeous site for getting refrence dictionaries! Thi
Dear Miri,
thanks a lot for looking after this! I would like to have the actual
reference dictionaries for the most frequent and important compounds in
PDB files: amino acids, desoxy-ribonucleotides and ribonucleotides.
If there is anything special in the convention about water, metal ions,
ha
Thurs. Feb. 11th 2010
EBI
Dear Dirk,
I see your point regarding the documentations, PDB is vast..
What exactly would you like to have?
Miri
On Thu, 11 Feb 2010, Dirk Kostrewa wrote:
Hello Miri,
yes, but if you look into these documents, they don't contain a good
dictionary of the usual com
Hello Miri,
yes, but if you look into these documents, they don't contain a good
dictionary of the usual compounds. They either have the broken link that
I've mentioned or point to the ftp-site with its cryptic collection.
Anyway, thanks for the pointer.
Best regards,
Dirk.
Am 11.02.10 10:
Dear Randy,
I've found this link already, but it is not very useful, because it only
generally describes the format. What is missing, is a good actual
dictionary of the usual compounds (amino acids, nucleic acids, and so
on). In the coordinate section, it only provides a link to the ftp-site
Thurs., feb. 11th 2010
EBI
Hello,
to add to Randy Read's note, the following URL has
http://www.wwpdb.org/docs.html
has this documment and others in PDF or HTML
Miri
On Thu, 11 Feb 2010, Randy Read wrote:
Dear Dirk,
By coincidence, I was talking on Tuesday to Jawahar Swaminathan (at PDBe
Dear Dirk,
By coincidence, I was talking on Tuesday to Jawahar Swaminathan (at PDBe at the
EBI), mentioning that I'd had trouble tracking down the documentation, and he
forwarded me a link:
http://www.wwpdb.org/documentation/format32/v3.2.html
Hopefully that has the information you need.
Rega
Dear CCP4ers,
I'm desperately trying to dig out a good documentation of the new PDB
3.2 nomenclature on www.wwpdb.org, but find myself running in circles.
There is even a broken link to the nomenclature file
http://remediation.wwpdb.org/downloads/Components-rel-alt.cif. The other
link, ftp://
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