Re: [ccp4bb] Nvidia 3D vision + 1920x1080 Desktop Displays

2010-03-11 Thread Christian Rausch
Dear Aidong, So what are your problems? Greetings, Christian Dr. Christian Rausch rau...@wzw.tum.de Lehrstuhl f. Biologische Chemie Phone: +49 (0)8161 71-4050 Technische Unive

Re: [ccp4bb] Nvidia 3D vision + 1920x1080 Desktop Displays

2010-03-11 Thread aidong
I would like to draw your attention: Do not rush to set these up now. There are many problems with quadro card and 3D vision kit running on linux. We are wasting a lot of time to work out solutions. Cheers Aidong On Mar 3, 2010, at 11:50 PM, Christian Rausch wrote: Hello, is someone u

[ccp4bb] March 15, 2010 deadline- User proposal submission for Collaborative Crystallography at BCSB

2010-03-11 Thread Banumathi Sankaran
Dear Users, The deadline for May/June 2010 Collaborative Crystallography proposals will be *Mar 15, 2010. * Through the Collaborative Crystallography Program (CC) at the Advanced Light Source (ALS), scientists can send

[ccp4bb] Graduate Course on Neutrons in Structural Biology - Dead line for application extended to March 31, 2010

2010-03-11 Thread Meilleur, Flora
Dear colleagues, We are organizing a course focusing on neutron techniques used in structural biology. The course is designed for graduate students with knowledge of protein function and structure but no or limited experience of neutron sciences. Date: June 7 - June 11, 2010 Location: Oak Ridg

Re: [ccp4bb] pdb file deposition

2010-03-11 Thread Kevin Jude
Probably, you have built water molecules that are associated with symmetry-related macromolecules rather than the host molecule. Turn symmetry on, check the nearest neighbors of the offending waters, and move the waters close to the host molecule if appropriate. I believe you can do this with the

[ccp4bb] pdb file deposition

2010-03-11 Thread Azadeh Shahsavar
Dear All, In depositing a pdb file, after validation step, an error comes up: *Solvent Atoms* The following solvent molecules lie farther than expected from the protein. Can any one give me some advice about it? deleting these water molecules results in a large increase of R factor, by the way.

Re: [ccp4bb] I 1 21 1 and C1 21 1 in CCP4

2010-03-11 Thread Ian Tickle
Hi Edward I don't understand, provided the MR program is doing its job, in this case there are no alternative choices for the space group; all the alternative space groups are equivalent and should refine equally well after MR - if they don't there's something badly wrong with your original data.

Re: [ccp4bb] I 1 21 1 and C1 21 1 in CCP4

2010-03-11 Thread Ian Tickle
Hi Edward There's no difference between any of the space groups you mention in terms of which is right or wrong, they are all equivalent: for the purposes of solving & refining the structure it makes absolutely no difference which one is chosen. A2, C2 and I2 differ only in the unit cell and the

[ccp4bb] I 1 21 1 and C1 21 1 in CCP4

2010-03-11 Thread Edward Snell
Dear All, I may be asking a dumb question and if so I apologize. I have a ~200 amino acid N-terminal 'arm' of a full protein (C-term already solved) that diffracts to ~2.1A. It integrates nicely in C2 and gives good molecular replacement models from Balbes in C121, 1I21, A121, C1211 and I1211 (

[ccp4bb] Crystallography course

2010-03-11 Thread Neela Yennawar
Dear All, Attached is information about a new X-ray crystallography training program being introduced at Pennsylvania State University. Kindly pass it along to anybody that may be interested in such an oppurtunity. Thank you Neela <>

Re: [ccp4bb] crystallization plates and robotics

2010-03-11 Thread Bob Cudney
Engin, Cc: ccp4bb The Innovaplate SD-2 (also known as the MRC 2 well plate, or MRC 2 Lens Crystallisation Microplate, or Swissci plate) is manufactured exclusively by Swissci (Switzerland) and distributed by several companies, including Hampton Research. The plate is manufactured and available

Re: [ccp4bb] crystallization plates and robotics

2010-03-11 Thread Annie Hassell
Engin-- We have used the Cybi-Well robot for daughtering from the deep well masterblocks into our crystallization trays for several years and really like it. It is made by Cy-Bio. HTH! annie Annie Hassell Glaxo Smithkline 5 Moore Drive RTP, NC 27709 919/483-3228 919/483-0368 (FAX) annie.

[ccp4bb] AW: [ccp4bb] Off-topic - Domain termini for construct design

2010-03-11 Thread alexander . pautsch
Dear Claudia, Though not a fixed rule, here are a few general things that we consider: - N- and C-termini of homologs (eg from BLAST) - Secondary structure predictions - Disorder prediction (eg disembl or grooms servers) - Limited proteolysis to experim

[ccp4bb] Help regarding installation of CCP4 package

2010-03-11 Thread Gowriishankar Raju
Dear all, I want to install the CCP4 package for our research studies in our lab system( 2GB RAM, 250 HD, windows server). But confused about the various versions like CCP4 for Linux OS and Windows OS . Please inform about the details of both versions. I have queries like, which one have all the m

Re: [ccp4bb] arp_warp 7.1 and CCP4 6.1.3

2010-03-11 Thread Victor Lamzin
Dear Pedro, This was fixed in ARP/wARP version 7.1 in January this year. We informed all who downloaded the software before the fix, but perhaps missed you. Just go now to www.arp-warp.org, download the package and install - everything should be fine. Best regards, Victor Pedro M. Matias

Re: [ccp4bb] crystallization plates and robotics

2010-03-11 Thread David Briggs
Hi, I have used the Art Robbins "hydra" to transfer reagents from deep well block to crystallisation plate, prior to using a mosquito to setup the drops. Assuming they are still available (this was about 4 yrs ago) the hydra was robust and easy to use. HTH, Dave -- Delivered via an Android. O

Re: [ccp4bb] arp_warp 7.1 and CCP4 6.1.3

2010-03-11 Thread Phil Evans
The attached modified install script strips off leading zeroes Phil (change name "-sh" to ".sh") install_csh-sh Description: Binary data On 11 Mar 2010, at 08:42, Pedro M. Matias wrote: > Hi. > > I don't know whether other people had this problem, but I've tried to install > arp_warp

Re: [ccp4bb] arp_warp 7.1 and CCP4 6.1.3

2010-03-11 Thread Tim Gruene
Oh dear, arp warp still has not learned counting then? On Thu, Mar 11, 2010 at 08:42:38AM +, Pedro M. Matias wrote: > Hi. > > I don't know whether other people had this problem, but I've tried to > install arp_warp 7.1 with CCP4 6.1.3 and the installation failed because > the refmac versi

Re: [ccp4bb] Off-topic - Domain termini for construct design

2010-03-11 Thread Tim Gruene
Hello Claudia, have you thought of, instead of rules, using restricted proteolysis combined with N-terminal sequencing in order to better estimate a good position at least for the N-terminal part of your protein? Tim On Thu, Mar 11, 2010 at 09:26:53AM +0100, Anastassis Perrakis wrote: > Hi - > >

[ccp4bb] arp_warp 7.1 and CCP4 6.1.3

2010-03-11 Thread Pedro M. Matias
Hi. I don't know whether other people had this problem, but I've tried to install arp_warp 7.1 with CCP4 6.1.3 and the installation failed because the refmac version is 5.5.0109. There's a conditional branch in the installation script that extracts the "0109" from this and does not like the l

Re: [ccp4bb] Off-topic - Domain termini for construct design

2010-03-11 Thread Anastassis Perrakis
Hi - I do not think there are "rules". There is a logic, to never truncate within secondary structure elements, and to prefer to delete regions that have a high probability to be disordered. With excuses for the non-ccp4 plugin, you can use our web server to help you design these. http: