Apologies for the non-crystallographic question, but I would think the wide
experience and vitality of this board might have some opinions on this
topic.
We've sent out some samples for negative staining electron microscopy. They
were stained with uranyl acetate on commercial grids and the result
On 15:52 Tue 25 May , Clayton, Gina Martyn wrote:
> I wonder if anyone can recommend a good review/paper describing
> crystal structures that show high energy residues in active sites. By
> that I mean residues that may be in a strained conformation and rotate
> between conformations, such t
Dear Ivan,
If I understand correctly what you want to achieve, one common way is to abuse
the B-factor column and replace those values with the values you want to
present.
Tim
On Thu, May 27, 2010 at 12:07:23PM -0700, xaravich ivan wrote:
> Hi,
> Apologies for a non CCP4 questions but this one i
Hi,
Apologies for a non CCP4 questions but this one is definately for
crystallographers,
I have a set of delta delta Gs for there different protein ligand
interactions.I want to show them in pyMOL. Is there any program or script
that I could run such that the output will be read by Pymol and color
Hi Jhon,
Here are a few hints for protein-DNA crystallization:
1.) Starting point for protein:DNA ratio is 1:1.2 for a tight binder. In
your case it would be protein complex to DNA 1:1.2. I like to run
size-exclusion chromatography (if applicable) to determine an optimal
ratio with a slight excess
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On Tue, May 25, 2010 at 6:58 AM, Pavel Afonine wrote:
> Yes, because all the information you need is encapsulated in 1 number
> per region of interest!
>
> I agree it's a good reason.
Actually what I described doesn't quite achieve this, because you need
to keep a record of both the RMSD Z-score
Hilgenberg (Malsfeld, Germany) currently offer their remaining stock.
Clemens
Zitat von James Holton :
Does anyone know the formula or supplier of what was once called
"Lindemann glass"? It is a silicon-free high-boron glass that has
no elements in it heavier than oxygen. Used to be used
Does anyone know the formula or supplier of what was once called
"Lindemann glass"? It is a silicon-free high-boron glass that has no
elements in it heavier than oxygen. Used to be used to make clear x-ray
windows, but now I can't find anyone who sells it.
-James Holton
MAD Scientist
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