[ccp4bb] Detergent and globular proteins

Dear all, We work with lipid droplet binding proteins in our group. These proteins behave more like membrane associated proteins, have a very hydrophobic surface and require detergents right from solubilization to crytallization. We use 1% DDM for solubilization, 0.03% DDM t

[ccp4bb] Postdoctoral positions available

Hi All, We are seeking highly motivated individuals to fill in postdoctoral positions to study structures and functions of proteins and protein complexes involved in assembly and genome packaging of complex DNA viruses such as human herpesviruses and bacteriophages by means of X-ray crystallo

Re: [ccp4bb] Crystal Caves Sun Oct 10 Nat Geo

There is a DVD available now by Juan-Manuel Garcia Ruiz. I previewed it at the ECM in Darmstadt - very impressive and better science than the Nat Geo flick. http://tinyurl.com/286su7w BR -Original Message- From: Bernhard Rupp (Hofkristallrat a.D.) [mailto:hofkristall...@gmail.com] Sent:

Re: [ccp4bb] Is the Rmerge invalidate by twinned data?

Fulvio, We need more info to give advice. First, when you say Rsym is 0.18, are you talking about in the high res bin or overall? Second, how did you determine you have twinning? In what space group did you scale your data? If your data is actually twinned with a high twin fraction, and yo

Re: [ccp4bb] Is the Rmerge invalidate by twinned data?

It may be time for our annual I/sigmaI discussion. Please note that is what the RCSB expects from you and it is generally lower than /. Some packages do not output in an obvious place for you to put in your Table 1. :) On Wed, 6 Oct 2010, Ed Pozharski wrote: You don't need twinning to

Re: [ccp4bb] Is the Rmerge invalidate by twinned data?

You don't need twinning to invalidate the Rmerge as a criterion for the resolution cutoff, there are other reasons why you should use I/sigma instead. If you process data all the way to 3A, what's the I/sigma in the highest resolution shell? On Wed, 2010-10-06 at 11:28 +0200, fulvio saccoccia wr

[ccp4bb] No TABFUN in Amore

Dear All, I have just installed the current CCP4 dmgs onto a Macbook pro, and everything seems to be OK. However, on running amore from ccp4i, in the initial sorting and tabling steps, the sorting works fine, but the tabling step does not run at all - i.e it is not called from the gui interfac

Re: [ccp4bb] Zalman

I recently got the 24 inch Zalman and when the coot window is maximized the 3D is reversed. I know I can minimize it slightly and move it up and down a pixel to fix it. Has anyone found a trick to leave it maximized and correct the 3D. I have tried adjusting screen position but have not foun

[ccp4bb] Is the Rmerge invalidate by twinned data?

Dear all, I have a data set collected at 3A resolution. I processed the data but I had to cut the resolution at 3.6A for the high value of Rmerge (at 3.6A it is 0.18). After scaling and MR I realized that my data were twinned. This is my question: can I reprocess all the data set using all the

Re: [ccp4bb] Zalman

On 01/10/10 16:57, Sickmier, Allen wrote: I recently got the 24 inch Zalman and when the coot window is maximized the 3D is reversed. I know I can minimize it slightly and move it up and down a pixel to fix it. Has anyone found a trick to leave it maximized and correct the 3D. I have tried

Re: [ccp4bb] stereo (again - sigh)

Hello, I am surprised to hear that it is difficult to get hold of Zalmans, so I spoke to a person from alternate this morning. At least people within Europe they are avaible and on stock (see www.alternate.eu) For people in the US, I am afraid, you would have to contact them directly - according

Re: [ccp4bb] Zalman

If u have external emitter controller then just press the "reverse" button .. thats how I solved this problem, but i use CRT monitor with Nvidia card in RHEL WS4. Cheers, Amit Das. -- --- AMIT DAS PROTEIN CRYSTALLOGRAPHY SO