Looks to me as if you have what we call ice rings, i.e. crystalline powder
diffraction. Plus what we call salt, i.e. strongly diffracting small molecule
crystal(s).
Fred.
Message du 20/02/11 14:47
De : Vandu Murugan
A : CCP4BB@JISCMAIL.AC.UK
Copie à :
Objet : [ccp4bb] Strage image
Hi
This is a powder diffraction pattern with a couple (or more) of small
molecule/salt crystals attached to it (which give rise to the spots).
The resolution of the rings isn't right for ice, so I'd guess they're
due to your precipitant forming small crystals.
If you have a sample with
But this wouldn't correspond to a globular form; 250kDa DNA will appear
larger than a 250kDa globular protein.
On Sat, Feb 19, 2011 at 5:25 PM, Artem Evdokimov
artem.evdoki...@gmail.comwrote:
A sufficiently long piece of DNA works OK.
On Sat, Feb 19, 2011 at 6:47 PM, Alexandra Deaconescu
On 02/20/11 08:46, Vandu Murugan wrote:
Dear All,
I mounted an aggregate with spikes on surface, that I saw in a
well, and got a image attached with this. It has concentric rings..
What it could be? Any comments on it...?
thanks,
- Wandu
Mentioning the temperature at which data was
PEG spherulite perhaps?
JPK
On Sun, Feb 20, 2011 at 7:46 AM, Vandu Murugan wandumuru...@gmail.com wrote:
Dear All,
I mounted an aggregate with spikes on surface, that I saw in a well,
and got a image attached with this. It has concentric rings.. What it
could be? Any comments on
If you notice, I did not say 250 kDa DNA. It will have to be a shorter DNA,
that *behaves* on size exclusion like a 250 kDa protein. I once worked out
the ratio, but don't remember what it ended up being, anymore :)
The advantage is that you can get these standards easily enough in a huge
range
Seeding ?
changing from sitting to hanging drop or under oil ?
play with temperature ?
Jürgen
On Feb 20, 2011, at 9:29 PM, Harvey Rodriguez wrote:
Dear CCP4BBers,
Recently I got a crystal which appeared in the condition 2.1M DL-Malic Acid, pH
7.0. The crystal looks like a dumbbell but was
Dear Harvey
Based on the experimental history you provided I would try two quick things in
order to obtain additional dimensions for your condition.
(1) set up your best consensus condition against an additive screen (e.g. From
Hampton).
(2) I would repeat your last pH vs precipitant grid
Dear harvey
Consider your purification and preparation protocols - is the sample pure and
homogenous?
Poul
On 21/02/2011, at 03.29, Harvey Rodriguez h.rodriguez.x...@gmail.com wrote:
Dear CCP4BBers,
Recently I got a crystal which appeared in the condition 2.1M DL-Malic Acid,
pH 7.0. The
