[ccp4bb] Job Vacancy: Instrument Scientist - Macromolecular Neutron Crystallography

2011-03-21 Thread Paul Henry
Instrument Scientist - Macromolecular Neutron Crystallography Description of position The Science Directorate at the European Spallation Source (ESS) in Lund, Sweden, invites applications for an Instrument Scientist, specialising in macromolecular single crystal crystallography. The successful

[ccp4bb] Maps after refmac twin refinement

2011-03-21 Thread Mary Ho
Does anyone know if the maps coefficients after twin refinement in refmac are b-sharpened? And if so, is the sharpening value output anywhere? Thanks, Mary X. Ho Postdoctoral Associate Arnold Lab Rutgers

Re: [ccp4bb] Data file wrangling question

2011-03-21 Thread Graeme Winter
Hi James, Indeed. I had not thought about that. I guess it would be helpful to have a list on a wiki page somewhere of task (i.e. changing dname for a file) and the list of tools that can do this. Perhaps when I get a quiet moment. Cheerio, Graeme On 18 March 2011 15:49, James Holton

Re: [ccp4bb] Maps after refmac twin refinement

2011-03-21 Thread Garib N Murshudov
Dear Mary It should not be unless you ask specifically using one of the following keywords mapc sharpen # automatically define sharpening parameters mapc sharpen bvalue # use this bvalue for sharpening with automatic regularisation parameter mapc sharpen alpha value # sharpen with

Re: [ccp4bb] Detergent/lipid crystal diffraction pattern?

2011-03-21 Thread Bjørn Panyella Pedersen
Working with the H+-ATPase AHA2 (C12E8/DDM/Cymal-5 detergent mixed micelle) we always saw a ring at ~40Å which we attributed to the micelle, but we have no experimental evidence that it was indeed caused by detergents. Heres a animated gif of this phenomenon:

Re: [ccp4bb] Detergent/lipid crystal diffraction pattern?

2011-03-21 Thread Maia Cherney
Hi PS What is the unit cell dimensions in the first crystal? It looks like protein to me. Maia On 21/03/2011 2:03 PM, Pius Padayatti wrote: Hi all, We recently observed some diffraction from membrane protein crystallization drops diffraction that look like non-proteinaceous (please see