Hi Pius
DS_1.png - protein diffraction !
DSA6E.png - surface ice formation and protein crystal which did not survive
the freezing !!
DSA2F.png - protein crystal which does not diffract, but cryo condition is
right !
Psp4f.png - internal ice formation plus some surface ice also protein
crystal
Pius,
Are you sure that you determined the correct cell. Which program did you
use? Usually there are much less spots on an image when a crystal has so
small unit size dimensions. To me the first crystal looks like protein.
Send it to a synchrotron and process the data in XDS. They can
Dear Crystallographers,
I have run my protein-peptide complex several times on a GE S200
10/300 in buffer A (below). Today, to make a crystallization stock, I
ran the sample in buffer B, and the peak shifted from a consistent
16.0 mL to 13.5mL, which would seem to be ~dimer MW, but I know that
At 07:23 PM 3/22/2011, Jacob Keller wrote:
Dear Crystallographers,
I have run my protein-peptide complex several times on a GE S200
10/300 in buffer A (below). Today, to make a crystallization stock, I
ran the sample in buffer B, and the peak shifted from a consistent
16.0 mL to 13.5mL, which
I was always told that gel filtration resins have a mild ion-exchange
character, hence the recommendation to use at least 100mM NaCl in size
exclusion buffers. Assuming that it is true, one would expect a protein to
stick to the resin in low salt buffers. That is the opposite of what you see
(your
Jacob,
Some protein can form weak dimer, especially in low salt buffer. AUC can
provide a more detailed info about your protein dimerization state.
Ray
On Mar 22, 2011, at 8:23 PM, Jacob Keller j-kell...@fsm.northwestern.edu
wrote:
Dear Crystallographers,
I have run my protein-peptide
Superdex 200 instruction manual suggests a minimal 150mM NaCl is
required to prevent binding of protein to the resin. But it seems more
to the side of preventing loss of protein instead of misjudging
protein size.
Nian Huang, Ph.D.
UT Southwestern Medical Center
On Tue, Mar 22, 2011 at 7:23 PM,
My 2 cents worth on the stereo-dependent:
1) They have carpal tunnel syndrome that makes it painful to keep the molecule
in motion while rebuilding it (NOTE: enough constant mouse-wiggling and you
will get carpal tunnel problems if you don't have them yet!)
2) They work on big, low-resolution
what about the fashion statement made by cool glasses?
From: Phoebe Rice [pr...@uchicago.edu]
Sent: Tuesday, March 22, 2011 10:16 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] while on the subject of stereo
My 2 cents worth on the stereo-dependent:
There are actually a bunch of depth cues that humans use to perceive
3-dimensionality. Existing computer displays only reproduce a few. Because of
redundancy, people can function with only a subset, but it can be a serious
handicap. Individuals who are stereoblind can learn to judge distances
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