The Hoelz laboratory at the California Institute of Technology seeks to recruit
an outstanding postdoctoral scientist in structural biology. Our laboratory
focuses on the structure-function relationships of multi-protein macromolecular
assemblies. Major research themes are (1) the nuclear pore
Hi Yogesh
I was probably the last person to update the SIGMAA source code, but I
have been using it without any problems. Could you post more info,
specifically the shell script you are using and an mtzdump listing of
the MTZ file header. If the problem can't be resolved from that info
I may
Dear all
I am refining a structure at 3.4 A resolution that contains 3 molecules in the
a.u. The chain A sits on a 2-fold crystallographic axis forming the dimeric
functional structure expected for this class of proteins. The other two chains
B and C, which also form the functional dimer, seem
Hi Fulvio
There are 2 different issues here: the Wilson plot scale B factor on
the one hand and Wilson statistics on the other. The first are not
affected by twinning since they depend only on the intensity averages
in shells. The second refers to the distribution of intensities (i.e.
the
Ok, so another question in my mind:
the data come from a collection interrupted after the first 200 frames
and restarted after 1 hour to recover the missed 160 frames.
The first and second group of frames, if separately indexed, give cell
parameter values that differ of about 1A for axes and 1
Hi Fulvio,
Yes, it can. An increase in cell parameters can indicate a failure of the
cooling system or (more likely) radiation damage of the crystal.
For refinement you probably do not need all those 360 frames,
unless you collected thin frames and your space group is P1
I suggest you integrate
Missing density could point to incomplete data, how is the completeness at high
and low resolution?
Sent from my iPhone
On May 19, 2011, at 11:54 AM, Jon Schuermann schue...@anl.gov wrote:
Dear Joane,
Bert's recommendations are very good, but I'd like to add a little
caution. If just
Max Planck Institute for Developmental Biology
Tuebingen, Germany.
PhD or postdoctoral positions funded by tax free fellowships are available
in the research group of Remco Sprangers and in the research group of
Silke Wiesner. We are looking for candidates that have a strong interest
This reminds me of:
Pig heart short chain L-3-hydroxyacyl-CoA dehydrogenase revisited:
Sequence analysis and crystal structure determination
Barycki JJ, O'Brien LK, Birktoft JJ, Strauss AW, Banaszak LJ
Protein Science (Oct 1999) Vol 8, pp 2010-2018.
In which the protein in question also had
Dear Joane,
We have had very good luck refining low resolution structures with dramatic
improvement using several newish options in refmac - particularly when NCS is
present! Those options are jelly body restraints, automatic NCS restraints,
and map sharpening. Here is a description
Applications are invited for a postdoctoral position in Dr. Fang
Li's
lab at the Department of Pharmacology, University of Minnesota Twin
Cities. Research involves biochemical and structural studies on
proteins that guide invasion and replication of important viral
pathogens. For more
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