Hi,
To purify your protein you can use GST-Trap HP column, it is quite easy, you
have to elute in a buffer with 20mM reduced glutathione.
HTH,
Catarina
--
Catarina Rodrigues, PhD Student
Molecular Transport & Signalling
AFMB UMR 6098 CNRS/UI/UII Case 932
163 Avenue de Luminy
13288 Marseille
Could my problem be because of switching from Windows_XP to Windows7???
Prof. Ylva Lindqvist
Dept. of Medical Biochemistry & Biophysics
Div. of Molecular Structural Biology
KAROLINSKA INSTITUTET
Tomtebodavägen 6
S-171 77 Stockholm, Sweden
Phone +46 8 52487695
Mobile +46 708 264356
Fax +46 8 32
After updating CCP4 includin Phaser_EP I can not run it - it can not read my
SIGFs - what is wrong? The error message is " SYNTAX ERROR: Use SIGFPOS SIGF(+)
or SIG+ " but my mtz-labels are LABIN F+ = F_Fe1(+) SIGF+ = SIGF_Fe1(+) F- =
F_Fe1(-) SIGF- = SIGF_Fe1(-). I have changed them with SFTOOLS
