[ccp4bb] PUI session at 2012 ACA meeting

2011-06-29 Thread Roger Rowlett
CCP4-ers, I am writing to alert the CCP4 community that I will be co-organizing (under the auspices of the Council on Undergraduate Research) a session at the 2012 American Crystallographic Association meeting (July 28-Aug 1) devoted to practical aspects

Re: [ccp4bb] Y-Chi2 running out of chart

2011-06-29 Thread Shiva Bhowmik
If you had that crystal saved after earlier collection then recollect a dataset again to see if the same Y-Chi2 profile is observed. On Mon, Jun 27, 2011 at 4:59 PM, bie gao wrote: > Jim, that's the first thing I tried - triclinic gave similar Y-Chi2 > profile. > Shiva, not sure what you mean by

[ccp4bb] The Good and the Bad crystal contact?

2011-06-29 Thread Paul Lindblom
Hi everybody, can anybody tell me how crystal contacts are defined? Are there good and bad crystal contacts? They are the most important interactions with impact on the crystal quality, but they are not of covalent nature, arenĀ“t they? With best regards, Paul

Re: [ccp4bb] The Good and the Bad crystal contact?

2011-06-29 Thread Michael Thompson
Hi Paul, While crystal contacts are typically of a non-covalent nature, there are some exceptions. A disulfide bond can act as a crystal contact, which is a covalent interaction. A technique called synthetic symmetrization involves the engineering of single cysteine mutants, followed by oxidati

Re: [ccp4bb] The Good and the Bad crystal contact?

2011-06-29 Thread Jacob Keller
What about the recently-published engineered DNA crystal: Nature. 2009 Sep 3;461(7260):74-7. >From molecular to macroscopic via the rational design of a self-assembled 3D DNA crystal. Zheng J, Birktoft JJ, Chen Y, Wang T, Sha R, Constantinou PE, Ginell SL, Mao C, Seeman NC. Source Department of Ch