[ccp4bb] Post-doctoral position available

2012-06-15 Thread Jim Spencer, Cellular and Molecular Medicine
Dear All, A postdoctoral position is available in my lab to study the interactions of zinc-dependent (metallo) beta-lactamases with beta-lactam substrates and candidate inhibitors using both rapid kinetics and X-ray crystallography. The position will be offered for one year in the first

Re: [ccp4bb] Question on Symmetry Axis Notation Convention

2012-06-15 Thread Ian Clifton
Ethan Merritt merr...@u.washington.edu writes: On Thursday, June 14, 2012 12:06:13 pm James Stroud wrote: Hello All, I would like to discuss symmetry axes, but I'm not sure what the notation convention is. For example, I'd like to say something about a 2(1) along the x-axis, but the phrase

Re: [ccp4bb] FFT for map variance

2012-06-15 Thread Ian Tickle
Frank, This has features in common with my proposal at the 2011 CCP4SW to use rho_obs / SU(rho_diff) as a model reliability (or precision) metric (now implemented in EDSTATS). This should not be confused with my proposed accuracy metric which uses a chi-square test based on the values of

Re: [ccp4bb] Off topic about application of detergent on non-membrane protein crystallization

2012-06-15 Thread Pius Padayatti
beta lactamase cymal 6 and see this reference Guan, R.-J., Wang, M., Liu, X.-Q, Wang, D.-C. Optimization of soluble protein crystallization with

Re: [ccp4bb] error popup with imosflm 1.0.6 despite valid executable MOSFLM_EXEC for mosflm 7.0.8

2012-06-15 Thread Harry Powell
Hi folks This problem arises because I inadvertently included the versions of MOSFLM_EXEC that require X11 libraries in the iMosflm distribution (for Linux only - Macs and Windows were okay) - iMosflm doesn't need these. I've put fixed binaries on the Mosflm download site for Linux -

Re: [ccp4bb] Off topic about application of detergent on non-membrane protein crystallization

2012-06-15 Thread mjvdwoerd
Hi Donghui, Yes. Crystals for restriction enzyme Bsob I were ugly and did not diffract well without detergent and were beautiful with good diffraction in presence of OBG. You can find it in the publication. We did not find any detergent molecules in the structure. Now, when to try such

Re: [ccp4bb] Off topic about application of detergent on non-membrane protein crystallization

2012-06-15 Thread Vitali Stanevich
Donghui, There is detergent screen from Hampton: http://hamptonresearch.com/product_detail.aspx?cid=1sid=39pid=31 We set up detergent screen for crystal conditions we'd like to improve: 200nl(buffer) + 200nl(protein) + 40nl(detergent). Occasionally (very occasionally) it works, but don't put to

[ccp4bb] Fw: [ccp4bb] Question on Symmetry Axis Notation Convention

2012-06-15 Thread REX PALMER
The standard notation is 21 axis parallel to [100]?   Rex Palmer http://www.bbk.ac.uk/biology/our-staff/emeritus-staff http://rexpalmer2010.homestead.com   From: James Stroud xtald...@gmail.com To: CCP4BB@JISCMAIL.AC.UK Sent: Thursday, 14 June 2012, 20:06