Dear all,
May I ask for recommendations for pH meters that work reliably over the range
of buffers and components that constitute common crystallization screens ?
Thanks very much in advance.
Anirban
Dear Tianlong,
you may use Arcimboldo using e.g. a long helix (or b-hairpin) as starting
model for de novo phasing. If you succeed to phase your structure, you
should be able see if it was part of your protein.
Best wishes
Kornelius
On Wed, Jul 18, 2012 at 6:46 AM, Tianlong Zhang wrote:
> **
Dear Tianlong,
You have probably crystallized a contaminant. You can check to see if your unit
cell has been seen before in the pdb with this service from Robert Esnouf at
Srubi,
http://www.strubi.ox.ac.uk/nearest-cell/nearest-cell.cgi
or apply to do a "wide search" whole pdb molecular replac
Oh, I know this one. It's called CNS :)
You probably have
to set
hydrogen_flag=true
in the generate.inp script - it's false by default. If some other
atoms are missing and you want to keep it that way, use
atom_build=(hydrogen)
Cheers,
Ed.
> Anyone know of a util that'll
add hydrogens
Anyone know of a util that'll add hydrogens in a naming scheme consistent with
CNS? (reduce doesn't do this).
Thanks!
F
-
Francis E. Reyes PhD
215 UCB
University of Colorado at Boulder
On Jul 17, 2012, at 6:25 AM, Felix Frolow wrote:
> I will wait for fink version if it will be one… :-\
Does anyone use or want this anymore?
-- Bill
William G. Scott
Professor
Department of Chemistry and Biochemistry
and The Center for the Molecular Biology of RNA
228 Sinsheimer Laboratories
try model-building with COOT,
I did it and my body is now totally flat (like beta-sheets) and stiff (like
alfa-helices)..--))
2012/7/17 Obayed Ullah
> http://www.acondicionamientos-sa.com/fevnzv.php?jrn=uoroea
>
--
Israel Sanchez Fernandez PhD
Ramakrishnan-lab
MRC Laboratory of Molecular Bi
Dear Georg,
You could have asked the beamline staff :). Transporting live viruses at LN2
temperatures is a problem and is the main reason for us only offering CL3
experiments for room temperature samples. I hadn't appreciated that you would
have the same problem with HG2 samples. Do you still h
http://www.acondicionamientos-sa.com/fevnzv.php?jrn=uoroea
Hi Wojtek,
attached is a simple Python script that will read in your EM map (in ccp4
format; I can trivially change the script so it reads x-plor map too) and
it will write out MTZ file containing:
- complex array of Fobs (Fobs_cmpl), which is just a Fourier transform of
the input map;
- real arr
Hi all,
an additional possibility would be to transport the crystals on dry ice.
This might be a completely stupid idea as the temperature is only -78°C.
But as dry ice shipment of such kind of material is less problematic and
transport material is available it would be fairly easy. And you ca
Hallo all,
The Biohazard Cryogenic Shipper EW-44300-22 looks really interesting. I
will contact cole-parmer. The problem might be that the shipper is
discontinued and the shipping time might be too long...
Thank you very much for your support,
Georg
Am 17.07.2012 20:42, schrieb Kris Tesh:
I would contact Cole-Parmer (http://www.coleparmer.com/) and see what they
recommend. They have been very helpful in the past.
Kris
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: Georg Zocher
To: CCP4BB@JISCMAIL.AC.
Dear Community,
I have to face the problem that I have to transport fully functional and
infectious virus crystals (frozen) to a synchrotron. The virus has
biosafety classification 2 (HSE group 2) and therefore transport by e.g.
Fedex has to fullfill the packing instruction 650, better 602 acc
Dear all,
i have got a .py file for the program *DeIce*.
However, i am unable to find a manual to incorporate it.
Can anyone suggest me how to use it in conjunction with HKL2000 or iMosflm.
Note: I have no experience using Python.
regards
--
Bhaba Krishna Das
Graduate Student
Structural and Com
For large unit cells, one must take particular care with the X-ray beam and the
orientation of the crystal. The latter has already been mentioned in previous
response. For the beam, some things to do are:
1. Make crystal smaller.
2. Make beam smaller (use a smaller collimator size).
3. Reduce b
We have also had success expressing two proteins separately and then mixing
the soluble fractions of the lysates in the presence of a
complex-initiating ligand, where one protein has a His-tag (preferably the
protein that expresses to a lower extent) and the other no affinity tag
followed by a Nick
On September 3-4 we are organizing a meeting in honor of Wim Hol:
Protein structure: from methods via structure and function to drug design.
The program ((http://xtal.nki.nl/Wim-Hol-Symposium) will consist of current
research of alumni from the Wim Hol lab in Groningen and Seattle, but
never
Lecturer/Senior lecturer position in X-ray crystallography located at the
Beatson Institute for Cancer Research.
The Beatson Institute for Cancer Research is committed to carry out world-class
research into the biology of cancer and to help develop better treatments to
improve the quality of lif
OOPS, I've made a big sign error in this "simple" calculation... Here are the
more correct results, script and revised conclusion:
With the correct effect of mosaicity:
dmin a* b* c*
4.01.36 1.17 0.48
3.00.93 0.79 0.27
2.00.51 0.41 0.07
1.00.0
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